Chassot Ana Lúcia Campani, Poisl Maria Inês Pereira, Samuel Susana Maria Werner
Department of Nursing and Dentistry, Faculty of Dentistry, University of Santa Cruz do Sul, Santa Cruz do Sul, RS, Brazil.
Braz Dent J. 2006;17(2):117-21. doi: 10.1590/s0103-64402006000200006.
The purpose of this study was to assess the antimicrobial efficacy of a peracetic acid-based disinfectant for decontamination of heat-polymerized, chemically activated and microwave-polymerized acrylic resins. Resin plates were contaminated in vivo upon intraoral use by 10 volunteers for 7 nights and slabs were contaminated in vitro by contact with Bacillus subtilis and Bacillus stearothermophilus. The contaminated acrylic resin specimens were immersed in a 0.2% peracetic acid-based disinfectant (Sterilife; Lifemed) for 5 min or 10 min and placed in a BHI culture medium. After incubation at 37 degrees C for 48 h, bacterial growth was assessed by analyzing turbidity of the medium. For all types of acrylic resin, no turbidity of the medium was observed for any of the resin specimens immersed in the peracetic acid-based disinfectant for either 5 or 10 min. On the other hand, the media with specimens that were not immersed in the disinfectant (control) showed turbidity in 100% of the cases, indicating the presence of microorganisms in both tested conditions. In conclusion, immersion for at least 5 min in a 0.2% peracetic acid-based disinfectant promoted high-level disinfection of heat-polymerized, chemically activated and microwave-polymerized acrylic resins contaminated with either human saliva or Bacillus subtilis or Bacillus stearothermophilus.
本研究的目的是评估一种过氧乙酸基消毒剂对热聚合、化学活化和微波聚合丙烯酸树脂进行去污处理的抗菌效果。树脂板在体内由10名志愿者口内使用7晚后被污染，平板在体外通过与枯草芽孢杆菌和嗜热脂肪芽孢杆菌接触而被污染。将受污染的丙烯酸树脂标本浸入0.2%的过氧乙酸基消毒剂（Sterilife；Lifemed）中5分钟或10分钟，然后置于脑心浸液培养基中。在37℃孵育48小时后，通过分析培养基的浊度来评估细菌生长情况。对于所有类型的丙烯酸树脂，浸入过氧乙酸基消毒剂5分钟或10分钟的任何树脂标本，培养基均未观察到浊度。另一方面，未浸入消毒剂的标本（对照）所在的培养基在100%的情况下均显示出浊度，表明在两种测试条件下均存在微生物。总之，将热聚合、化学活化和微波聚合的丙烯酸树脂用0.2%的过氧乙酸基消毒剂浸泡至少5分钟，可有效对被人唾液、枯草芽孢杆菌或嗜热脂肪芽孢杆菌污染的树脂进行高水平消毒。
