Fry John C, Webster Gordon, Cragg Barry A, Weightman Andrew J, Parkes R John
Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK.
FEMS Microbiol Ecol. 2006 Oct;58(1):86-98. doi: 10.1111/j.1574-6941.2006.00144.x.
The aim of this work was to relate depth profiles of prokaryotic community composition with geochemical processes in the deep subseafloor biosphere at two shallow-water sites on the Peru Margin in the Pacific Ocean (ODP Leg 201, sites 1228 and 1229). Principal component analysis of denaturing gradient gel electrophoresis banding patterns of deep-sediment Bacteria, Archaea, Euryarchaeota and the novel candidate division JS1, followed by multiple regression, showed strong relationships with prokaryotic activity and geochemistry (R(2)=55-100%). Further correlation analysis, at one site, between the principal components from the community composition profiles for Bacteria and 12 other variables quantitatively confirmed their relationship with activity and geochemistry, which had previously only been implied. Comparison with previously published cell counts enumerated by fluorescent in situ hybridization with rRNA-targeted probes confirmed that these denaturing gradient gel electrophoresis profiles described an active prokaryotic community.
本研究的目的是将太平洋秘鲁边缘两个浅水站点（大洋钻探计划第201航次，1228和1229站点）深海海底生物圈中原核生物群落组成的深度剖面与地球化学过程联系起来。对深部沉积物中的细菌、古菌、广古菌门和新的候选类群JS1的变性梯度凝胶电泳条带模式进行主成分分析，随后进行多元回归分析，结果表明其与原核生物活性和地球化学之间存在密切关系（R² = 55 - 100%）。在一个站点，对细菌群落组成剖面的主成分与其他12个变量进行进一步的相关性分析，定量证实了它们与活性和地球化学之间的关系，而这种关系此前只是一种推测。与之前发表的使用rRNA靶向探针进行荧光原位杂交计数的细胞数量进行比较，证实这些变性梯度凝胶电泳剖面描述的是一个活跃的原核生物群落。
