Brichacek Beda, Vanpouille Christophe, Trachtenberg Alexander J, Pushkarsky Tatiana, Dubrovsky Larisa, Martin Gregory, Simon Gary, Bukrinsky Michael
Department of Microbiology, Immunology and Tropical Medicine, George Washington University Medical Center, Washington, DC, USA.
BMC Immunol. 2006 Sep 11;7:21. doi: 10.1186/1471-2172-7-21.
Members of the United States Armed Forces receive a series of vaccinations during their course of service. To investigate the influence of multiple vaccinations on innate immunity, we measured concentrations of a panel of immunomodulatory and pro-inflammatory cytokines in serum samples from a group of such individuals.
Significantly increased levels of macrophage inflammatory protein 1alpha (MIP-1alpha), MIP-1beta and interleukin 8 (IL-8) were detected. Since these cytokines are known to have anti-human immunodeficiency virus (HIV) activity, we tested the effect of serum from these individuals on HIV-1 infectivity and susceptibility of their peripheral blood mononuclear cells (PBMCs) to HIV-1 infection in vitro. Sera from vaccinated military personnel inhibited, and their PBMCs were partially resistant to, infection by HIV-1 strains tropic to CCR5 (R5), but not to CXCR4 (X4), chemokine receptor.
These findings demonstrate that increased anti-HIV chemokines can be detected in vaccine recipients up to 68 weeks following immunization.
美国武装部队成员在服役期间会接受一系列疫苗接种。为了研究多次接种疫苗对先天免疫的影响，我们测量了一组此类个体血清样本中一系列免疫调节和促炎细胞因子的浓度。
检测到巨噬细胞炎性蛋白1α（MIP-1α）、MIP-1β和白细胞介素8（IL-8）水平显著升高。由于已知这些细胞因子具有抗人类免疫缺陷病毒（HIV）活性，我们在体外测试了这些个体的血清对HIV-1感染性及其外周血单个核细胞（PBMC）对HIV-1感染易感性的影响。接种疫苗的军事人员的血清可抑制对CCR5（R5）趋化因子受体有嗜性的HIV-1毒株的感染，并且他们的PBMC对该毒株部分具有抗性，但对CXCR4（X4）趋化因子受体有嗜性的毒株没有抗性。
这些发现表明，在免疫接种后长达68周的疫苗接种者中可检测到抗HIV趋化因子增加。
