da Rosa Elizabeth S T, Kotait Ivanete, Barbosa Taciana F S, Carrieri Maria L, Brandão Paulo E, Pinheiro Amiraldo S, Begot Alberto L, Wada Marcelo Y, de Oliveira Rosely C, Grisard Edmundo C, Ferreira Márcia, Lima Reynaldo J da Silva, Montebello Lúcia, Medeiros Daniele B A, Sousa Rita C M, Bensabath Gilberta, Carmo Eduardo H, Vasconcelos Pedro F C
Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministry of Health, Av. Almirante Barroso 492, 66093-020 Belém, Brazil.
Emerg Infect Dis. 2006 Aug;12(8):1197-202. doi: 10.3201/eid1208.050929.
We describe 2 bat-transmitted outbreaks in remote, rural areas of Portel and Viseu Municipalities, Pará State, northern Brazil. Central nervous system specimens were taken after patients' deaths and underwent immunofluorescent assay and histopathologic examination for rabies antigens; also, specimens were injected intracerebrally into suckling mice in an attempt to isolate the virus. Strains obtained were antigenically and genetically characterized. Twenty-one persons died due to paralytic rabies in the 2 municipalities. Ten rabies virus strains were isolated from human specimens; 2 other cases were diagnosed by histopathologic examination. Isolates were antigenically characterized as Desmodus rotundus variant 3 (AgV3). DNA sequencing of 6 strains showed that they were genetically close to D. rotundus-related strains isolated in Brazil. The genetic results were similar to those obtained by using monoclonal antibodies and support the conclusion that the isolates studied belong to the same rabies cycle, the virus variants found in the vampire bat D. rotundus.
我们描述了巴西北部帕拉州波特莱和维塞乌市偏远农村地区发生的两起由蝙蝠传播的疫情。患者死亡后采集了中枢神经系统标本，并进行了狂犬病抗原的免疫荧光检测和组织病理学检查；此外，还将标本脑内接种到乳鼠体内以尝试分离病毒。对获得的毒株进行了抗原和基因特征分析。这两个市有21人死于麻痹型狂犬病。从人类标本中分离出10株狂犬病病毒；另外2例通过组织病理学检查确诊。分离株抗原性鉴定为圆叶叶口蝠变种3（AgV3）。对6株病毒的DNA测序表明，它们在基因上与在巴西分离的与圆叶叶口蝠相关的毒株相近。基因检测结果与使用单克隆抗体获得的结果相似，支持了所研究的分离株属于同一狂犬病传播周期的结论，即吸血蝙蝠圆叶叶口蝠中发现的病毒变种。
