Li Feng-Hua, Liu Ping, Xiong Wei-Guo, Xu Guang-Fu
Institute of Liver Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhong Xi Yi Jie He Xue Bao. 2006 Sep;4(5):514-7. doi: 10.3736/jcim20060515.
To study the effects of Cordyceps sinensis on dimethylnitrosamine-induced liver fibrosis in rats.
SD rats were divided into normal control group, untreated group and Cordyceps sinensis-treated group. The rats in each group were fed with corresponding drug for 4 weeks. The rat's liver collagen deposition was observed with collagen staining. Hydroxyproline (Hyp) contents in liver tissue of the rats in 3 groups were determined with HCl hydrolysis. The tissue inhibitor of metalloproteinase-2 (TIMP-2) and type IV collagen contents were observed by Envision, and matrix metalloproteinases-2 (MMP-2) activity was detected by the method of enzyme-picture. Type I collagen was detected by Western blotting.
The contents of Hyp, TIMP-2, type IV collagen, and the expression of type I collagen in untreated group were significantly higher than those in the normal control group, while those in Cordyceps sinensis-treated group were significantly lower than those in the untreated group. The content of MMP-2 in untreated group was significantly lower than that in the normal control group, while that in Cordyceps sinensis-treated group was significantly higher than that in the untreated group.
Cordyceps sinensis can considerably relieve the liver fibrosis, and the mechanism may be related to promoting the degradation of the collagens.
研究冬虫夏草对二甲基亚硝胺诱导的大鼠肝纤维化的影响。
将SD大鼠分为正常对照组、未治疗组和冬虫夏草治疗组。每组大鼠给予相应药物喂养4周。采用胶原染色观察大鼠肝脏胶原沉积情况。用盐酸水解法测定3组大鼠肝组织中羟脯氨酸（Hyp）含量。采用Envision法观察金属蛋白酶组织抑制因子-2（TIMP-2）和IV型胶原含量，用酶谱法检测基质金属蛋白酶-2（MMP-2）活性。采用蛋白质印迹法检测I型胶原。
未治疗组Hyp、TIMP-2、IV型胶原含量及I型胶原表达均显著高于正常对照组，而冬虫夏草治疗组均显著低于未治疗组。未治疗组MMP-2含量显著低于正常对照组，而冬虫夏草治疗组显著高于未治疗组。
冬虫夏草可显著减轻肝纤维化，其机制可能与促进胶原降解有关。
