冬虫夏草对慢性肾病大鼠模型的肝脏和心脏损伤具有保护作用：一项代谢组学分析。
Cordyceps sinensis protects against liver and heart injuries in a rat model of chronic kidney disease: a metabolomic analysis.
作者信息
Liu Xia, Zhong Fang, Tang Xu-long, Lian Fu-lin, Zhou Qiao, Guo Shan-mai, Liu Jia-fu, Sun Peng, Hao Xu, Lu Ying, Wang Wei-ming, Chen Nan, Zhang Nai-xia
机构信息
Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Department of Nephrology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China.
出版信息
Acta Pharmacol Sin. 2014 May;35(5):697-706. doi: 10.1038/aps.2013.186. Epub 2014 Mar 17.
AIM
To test the hypothesis that the traditional Chinese medicine Cordyceps sinensis could improve the metabolic function of extrarenal organs to achieve its anti-chronic kidney disease (CKD) effects.
METHODS
Male SD rats were divided into CKD rats (with 5/6-nephrectomy), CKD rats treated with Cordyceps sinensis (4 mg•kg-1•d-1, po), and sham-operated rats. After an 8-week treatment, metabolites were extracted from the hearts and livers of the rats, and then subjected to (1)H-NMR-based metabolomic analysis.
RESULTS
Oxidative stress, energy metabolism, amino acid and protein metabolism and choline metabolism were considered as links between CKD and extrarenal organ dysfunction. Within the experimental period of 8 weeks, the metabolic disorders in the liver were more pronounced than in the heart, suggesting that CKD-related extrarenal organ dysfunctions occurred sequentially rather than simultaneously. Oral administration of Cordyceps sinensis exerted statistically significant rescue effects on the liver and heart by reversely regulating levels of those metabolites that are typically perturbed in CKD.
CONCLUSION
Oral administration of Cordyceps sinensis significantly attenuates the liver and heart injuries in CKD rats. The (1)H NMR-based metabolomic approach has provided a systematic view for understanding of CKD and the drug treatment, which can also be used to elucidate the mechanisms of action of other traditional Chinese medicines.
目的
验证中药冬虫夏草可改善肾外器官代谢功能以实现其抗慢性肾脏病（CKD）作用的假说。
方法
将雄性SD大鼠分为CKD大鼠（行5/6肾切除术）、冬虫夏草治疗的CKD大鼠（4 mg•kg-1•d-1，口服）和假手术大鼠。经过8周治疗后，从大鼠的心脏和肝脏中提取代谢物，然后进行基于氢核磁共振（1H-NMR）的代谢组学分析。
结果
氧化应激、能量代谢、氨基酸和蛋白质代谢以及胆碱代谢被认为是CKD与肾外器官功能障碍之间的联系。在8周的实验期内，肝脏中的代谢紊乱比心脏中更明显，这表明与CKD相关的肾外器官功能障碍是相继发生而非同时出现的。口服冬虫夏草通过反向调节CKD中通常受到干扰的那些代谢物的水平，对肝脏和心脏产生了具有统计学意义的挽救作用。
结论
口服冬虫夏草可显著减轻CKD大鼠的肝脏和心脏损伤。基于1H NMR的代谢组学方法为理解CKD和药物治疗提供了系统的视角，也可用于阐明其他中药的作用机制。
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