Heacock C S, Sutherland R M
Experimental Therapeutics Division of the Cancer Center, University of Rochester, School of Medicine and Dentistry, NY 14642.
Br J Cancer. 1990 Aug;62(2):217-25. doi: 10.1038/bjc.1990.264.
Cultured cells maintained in very low oxygen levels alter their structure, metabolism and genetic expression. Culture conditions for cells were modified to minimise variation of nutrients and to allow normal survival levels after 24 h of hypoxic exposure. Under these hypoxic conditions, glucose consumption and lactate production rates were similar to aerobic rates until about 12 h after which the hypoxic rates increased. DNA and protein synthesis rates are continuously inhibited to about 48% or 55% of the respective aerobic rates. During this period of decreased protein synthesis, a set of proteins termed oxygen regulated proteins (ORPs), exhibits enhanced relative synthesis. The molecular weights of the five major ORPs are approximately 260, 150, 100, 80 and 33 kDa. While increased relative synthesis of oxygen regulated proteins is partly due to increased levels of mRNA which encode these proteins, the mechanism of enhanced synthesis of ORPs may be more complex.
在极低氧水平下培养的细胞会改变其结构、代谢和基因表达。对细胞培养条件进行了修改，以尽量减少营养物质的变化，并使细胞在缺氧暴露24小时后能保持正常存活水平。在这些缺氧条件下，葡萄糖消耗率和乳酸产生率在约12小时前与有氧条件下的速率相似，之后缺氧条件下的速率增加。DNA和蛋白质合成速率持续受到抑制，分别约为各自有氧条件下速率的48%或55%。在蛋白质合成减少的这段时间里，一组被称为氧调节蛋白（ORPs）的蛋白质表现出相对合成增强。五种主要ORPs的分子量约为260、150、100、80和33 kDa。虽然氧调节蛋白相对合成增加部分是由于编码这些蛋白质的mRNA水平升高，但ORPs合成增强的机制可能更为复杂。
