Chen An-Ping, Wang Gui-Ling, Qu Zhan-Liang, Lu Chun-Xia, Liu Ning, Wang Fang, Xia Gui-Xian
National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Plant Cell Rep. 2007 Feb;26(2):237-45. doi: 10.1007/s00299-006-0238-y. Epub 2006 Sep 14.
The halophyte Thellungiella halophila (salt cress) is an ideal model system for studying the molecular mechanisms of salinity tolerance in plants. Herein, we report the identification of a stress-responsive cyclophilin gene (ThCYP1) from T. halophila, using fission yeast as a functional system. The expression of ThCYP1 is highly inducible by salt, abscisic acid (ABA), H(2)O(2) and heat shock. Ectopic overexpression of the ThCYP1 gene enhance the salt tolerance capacity of fission yeast and tobacco (Nicotiana tabacum L.) cv. Bright Yellow 2 (BY-2) cells significantly. ThCYP1 is expressed constitutively in roots, stems, leaves and flowers, with higher expression occurring in the roots and flowers. The ThCYP1 proteins are distributed widely within the cell, but are enriched significantly in the nucleus. The present results suggest that ThCYP1 may participate in response to stresses in the salt cress, perhaps by regulating appropriate folding of certain stress-related proteins, or in the signal transduction processes.
盐生植物盐芥（Thellungiella halophila）是研究植物耐盐分子机制的理想模式系统。在此，我们报道了利用裂殖酵母作为功能系统，从盐芥中鉴定出一个胁迫响应亲环素基因（ThCYP1）。ThCYP1的表达受盐、脱落酸（ABA）、H₂O₂和热激高度诱导。ThCYP1基因的异位过表达显著增强了裂殖酵母和烟草（Nicotiana tabacum L.）品种亮黄2（BY-2）细胞的耐盐能力。ThCYP1在根、茎、叶和花中组成型表达，在根和花中的表达较高。ThCYP1蛋白在细胞内广泛分布，但在细胞核中显著富集。目前的结果表明，ThCYP1可能参与盐芥对胁迫的响应，也许是通过调节某些胁迫相关蛋白的正确折叠，或参与信号转导过程。
