Debacq Christophe, Gillet Nicolas, Asquith Becca, Sanchez-Alcaraz Maria Teresa, Florins Arnaud, Boxus Mathieu, Schwartz-Cornil Isabelle, Bonneau Michel, Jean Geneviève, Kerkhofs Pierre, Hay Jack, Théwis André, Kettmann Richard, Willems Luc
Molecular and Cellular Biology, FNRS-FUSAG, Gembloux, Belgium.
J Virol. 2006 Oct;80(19):9710-9. doi: 10.1128/JVI.01022-06.
The size of a lymphocyte population is primarily determined by a dynamic equilibrium between cell proliferation and death. Hence, lymphocyte recirculation between the peripheral blood and lymphoid tissues is a key determinant in the maintenance of cell homeostasis. Insights into these mechanisms can be gathered from large-animal models, where lymphatic cannulation from individual lymph nodes is possible. In this study, we assessed in vivo lymphocyte trafficking in bovine leukemia virus (BLV)-infected sheep. With a carboxyfluorescein diacetate succinimidyl ester labeling technique, we demonstrate that the dynamics of lymphocyte recirculation is unaltered but that accelerated proliferation in the lymphoid tissues is compensated for by increased death in the peripheral blood cell population. Lymphocyte homeostasis is thus maintained by biphasic kinetics in two distinct tissues, emphasizing a very dynamic process during BLV infection.
淋巴细胞群体的大小主要由细胞增殖与死亡之间的动态平衡决定。因此，外周血与淋巴组织之间的淋巴细胞再循环是维持细胞稳态的关键决定因素。对这些机制的深入了解可从大型动物模型中获得，在这些模型中，可对单个淋巴结进行淋巴管插管。在本研究中，我们评估了牛白血病病毒（BLV）感染绵羊体内的淋巴细胞运输情况。通过羧基荧光素二乙酸琥珀酰亚胺酯标记技术，我们证明淋巴细胞再循环的动力学未改变，但淋巴组织中加速的增殖被外周血细胞群体中增加的死亡所补偿。因此，淋巴细胞稳态通过两个不同组织中的双相动力学得以维持，这强调了BLV感染期间一个非常动态的过程。
