Kazda J, Stackebrandt E, Smida J, Minnikin D E, Daffe M, Parlett J H, Pitulle C
Division of Veterinary Medical Microbiology, Research Institute Borstel, Federal Republic of Germany.
Int J Syst Bacteriol. 1990 Jul;40(3):217-23. doi: 10.1099/00207713-40-3-217.
Strains of a new type of slowly growing scotochromogenic mycobacterium were isolated repeatedly from sphagnum vegetation and surface water of moors in New Zealand. These strains grew at 31 and 22 degrees C but not at 37 degrees C and possessed catalase, acid phosphatase, and arylsulfatase activities. They did not split amides, and most of them were susceptible to antituberculotic drugs. Furthermore, they did not tolerate 0.1% NaOH2 and 0.2% picric acid and did not grow on compounds used as single carbon sources and single nitrogen and carbon sources. The internal similarity of the strains as determined by numerical taxonomy methods was 96.6% +/- 3.09%. The whole-mycolate pattern is unique in that it has not been found previously in 23 species of slowly growing mycobacteria. Evaluation of long-reverse-transcriptase-generated stretches of the primary structure of the 16S rRNA confirmed that these organisms belong to the genus Mycobacterium. The phylogenetic position of these bacteria is unique; they are situated between slowly growing pathogenic and rapidly growing saprophytic species. The strains are not pathogenic for mice, guinea pigs, and rabbits, but they provoke a nonspecific hypersensitivity reaction to bovine tuberculin. Hence, they are considered members of a new species of nonpathogenic, slowly growing mycobacteria, for which the name Mycobacterium cookii is proposed. Strain NZ2 is the type strain; a culture of this strain has been deposited in the American Type Culture Collection as strain ATCC 49103.
一种新型缓慢生长的暗产色分枝杆菌菌株，多次从新西兰泥炭藓植被和泥炭地地表水分离得到。这些菌株在31℃和22℃生长，但在37℃不生长，具有过氧化氢酶、酸性磷酸酶和芳基硫酸酯酶活性。它们不分解酰胺，大多数对抗结核药物敏感。此外，它们不耐受0.1%氢氧化钠和0.2%苦味酸，不能在用作单一碳源、单一氮源和碳源的化合物上生长。通过数值分类学方法确定的菌株内部相似性为96.6%±3.09%。全分枝菌酸模式独特，因为在23种缓慢生长的分枝杆菌中以前未发现过。对16S rRNA一级结构的长反转录酶生成片段的评估证实，这些微生物属于分枝杆菌属。这些细菌的系统发育位置独特；它们位于缓慢生长的致病菌种和快速生长的腐生菌种之间。这些菌株对小鼠、豚鼠和兔子无致病性，但会引发对牛结核菌素的非特异性超敏反应。因此，它们被认为是一种新的非致病性、缓慢生长分枝杆菌的成员，为此提出了库克分枝杆菌这一名称。菌株NZ2是模式菌株；该菌株的培养物已作为菌株ATCC 49103保藏于美国典型培养物保藏中心。
