Kazda J, Cooney R, Monaghan M, Quinn P J, Stackebrandt E, Dorsch M, Daffé M, Müller K, Cook B R, Tarnok Z S
Research Institute for Experimental Biology and Medicine, Borstel, Germany.
Int J Syst Bacteriol. 1993 Apr;43(2):352-7. doi: 10.1099/00207713-43-2-352.
Strains of a new type of slowly growing scotochromogenic, rose-pink-pigmented mycobacterium were isolated repeatedly from sphagnum vegetation, true moss, and soil in Ireland. These strains grew at 22, 31, and 37 degrees C but not at 45 degrees C and possessed acid phosphatase and arylsulfatase activities. They reduced nitrate, tolerated 0.1% NaNO2, did not split amides, and were resistant to most of the antituberculous drugs tested, except ethambutol. They did not form acid from glucose and mannose. Their internal phenetic similarity was 97.08% +/- 2.07%. The whole mycolate pattern confirmed the homogeneity of the taxa sharing similar mycolate types with several other mycobacterial species. However, on the basis of the nature of the major pyrolysis esters, the taxon appeared unique. The phylogenetic analysis based on evolutionary distance values revealed that the strains belong to a new species of slowly growing mycobacteria. The DNA-DNA hybridization values confirmed that these strains differ significantly from Mycobacterium nonchromogenicum, M. terrae, M. triviale, and M. thermoresistibile. The strains produced a unique rose-pink pigment and were nonpathogenic for mice, guinea pigs, and rabbits, but they provoked a nonspecific hypersensitivity reaction to bovine tuberculin in guinea pigs and cattle. Hence, they are considered a member of a new species of nonpathogenic slowly growing mycobacteria, for which the name Mycobacterium hiberniae is proposed. Strain Hi 11 is the type strain, a culture of which has been deposited in the American Type Culture Collection as strain ATCC 49874.
一种新型的生长缓慢的暗产色、玫瑰粉色色素的分枝杆菌菌株，在爱尔兰的泥炭藓植被、真藓类植物和土壤中被反复分离出来。这些菌株在22℃、31℃和37℃下生长，但在45℃下不生长，并且具有酸性磷酸酶和芳基硫酸酯酶活性。它们能还原硝酸盐，耐受0.1%的亚硝酸钠，不分解酰胺，并且对除乙胺丁醇外的大多数测试抗结核药物具有抗性。它们不能从葡萄糖和甘露糖产酸。它们的内部表型相似性为97.08%±2.07%。整个霉菌酸模式证实了与其他几种分枝杆菌物种具有相似霉菌酸类型的分类群的同质性。然而，基于主要热解酯的性质，该分类群显得独特。基于进化距离值的系统发育分析表明，这些菌株属于一种新的生长缓慢的分枝杆菌物种。DNA-DNA杂交值证实这些菌株与非产色分枝杆菌、地分枝杆菌、琐事分枝杆菌和耐热分枝杆菌有显著差异。这些菌株产生独特的玫瑰粉色色素，对小鼠、豚鼠和兔子无致病性，但它们在豚鼠和牛中引发了对牛结核菌素的非特异性超敏反应。因此，它们被认为是一种新的非致病性生长缓慢的分枝杆菌物种的成员，为此提出了“爱尔兰分枝杆菌”这一名称。菌株Hi 11是模式菌株，其培养物已作为菌株ATCC 49874保藏于美国典型培养物保藏中心。
