Nieman David C, Henson Dru A, Davis J Mark, Dumke Charles L, Utter Alan C, Murphy E Angela, Pearce Steven, Gojanovich Greg, McAnulty Steven R, McAnulty Lisa S
Department of Health, Leisure, and Exercise Science, Biology, Family and Consumer Sciences, Fischer Hamilton/Nycom Biochemistry Laboratory, Appalachian State University, Boone, NC 28608, USA.
J Interferon Cytokine Res. 2006 Sep;26(9):668-74. doi: 10.1089/jir.2006.26.668.
The primary purpose of this project was to study exercise-induced leukocyte cytokine mRNA expression. Changes in plasma cytokine levels and blood leukocyte mRNA expression for interleukin-6 (IL-6), IL-8, IL- 10, and IL-1 receptor antagonist (IL-1Ra) were measured in 12 athletes following 2 h of intensive cycling ( approximately 64% Watts(max)) while ingesting a carbohydrate or placebo beverage (randomized and double blinded). Blood samples were collected 30 min preexercise and immediately and 1 h postexercise. Carbohydate compared with placebo ingestion attenuated exercise-induced changes in plasma cortisol (8.8% vs. 62%, respectively), epinephrine (-9.2% vs. 138%), IL-6 (10-fold vs. 40-fold), IL-10 (8.9-fold vs. 26-fold, and IL-1Ra (2.1-fold vs. 5.6-fold). Significant time effects were measured for blood leukocyte IL-8 (2.4-fold increase 1 h postexercise), IL-10 (2.7-fold increase), IL-1Ra (2.2-fold increase), and IL-6 (0.8-fold decrease) mRNA content, with no significant differences between Cho and Pla test conditions. In summary, gene expression for IL-8, IL-10, and IL-1Ra, but not IL-6, is increased in blood leukocytes taken from athletes following 2 h of intensive cycling and is not influenced by carbohydrate compared with placebo ingestion. mRNA expression was high enough to indicate a substantial contribution of blood leukocytes to plasma levels of IL-8, IL-10, and IL-1Ra during prolonged exercise.
本项目的主要目的是研究运动诱导的白细胞细胞因子mRNA表达。在12名运动员进行2小时高强度骑行（约64%最大功率）期间，分别摄入碳水化合物饮料或安慰剂饮料（随机双盲），测定血浆细胞因子水平以及白细胞中白细胞介素-6（IL-6）、IL-8、IL-10和白细胞介素-1受体拮抗剂（IL-1Ra）的mRNA表达变化。在运动前30分钟、运动后即刻和运动后1小时采集血样。与摄入安慰剂相比，摄入碳水化合物可减轻运动诱导的血浆皮质醇（分别为8.8%和62%）、肾上腺素（-9.2%和138%）、IL-6（10倍和40倍）、IL-10（8.9倍和26倍）以及IL-1Ra（2.1倍和5.6倍）的变化。测定了血白细胞IL-8（运动后1小时增加2.4倍）、IL-10（增加2.7倍）、IL-1Ra（增加2.2倍）和IL-6（降低0.8倍）mRNA含量的显著时间效应，在碳水化合物组和安慰剂组测试条件之间无显著差异。总之，在进行2小时高强度骑行的运动员的血白细胞中，IL-8、IL-10和IL-1Ra的基因表达增加，而IL-6未增加，与摄入安慰剂相比，摄入碳水化合物对此无影响。mRNA表达水平高到足以表明在长时间运动期间血白细胞对血浆IL-8、IL-10和IL-1Ra水平有显著贡献。
