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PsJN 对葡萄酚类物质代谢的影响。

Impact of PsJN on Grapevine Phenolic Metabolism.

机构信息

Unité de Recherche EA 4707 Résistance Induite et Bioprotection des Plantes (RIBP), Université de Reims Champagne-Ardenne, SFR Condorcet FR CNRS 3417, 51687 Reims Cedex 2, France.

Ecologie Microbienne, Université Lyon 1, CNRS, INRA, UMR 5557, 69622 Villeurbanne, France.

出版信息

Int J Mol Sci. 2019 Nov 16;20(22):5775. doi: 10.3390/ijms20225775.

DOI:10.3390/ijms20225775
PMID:31744149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6888286/
Abstract

Phenolic compounds are implied in plant-microorganisms interaction and may be induced in response to plant growth-promoting rhizobacteria (PGPRs). Among PGPR, the beneficial bacterium PsJN was previously described to stimulate the growth of plants and to induce a better adaptation to both abiotic and biotic stresses. This study aimed to investigate the impact of PsJN on grapevine secondary metabolism. For this purpose, gene expression (qRT-PCR) and profiling of plant secondary metabolites (UHPLC-UV/DAD-MS QTOF) from both grapevine root and leaves were compared between non-bacterized and PsJN-bacterized grapevine plantlets. Our results showed that PsJN induced locally (roots) and systemically (leaves) an overexpression of and and specifically in leaves the overexpression of all the genes implied in phenylpropanoid and flavonoid pathways. Moreover, the metabolomic approach revealed that relative amounts of 32 and 17 compounds in roots and leaves, respectively, were significantly modified by PsJN. Once identified to be accumulated in response to PsJN by the metabolomic approach, antifungal properties of purified molecules were validated in vitro for their antifungal effect on spore germination. Taking together, our findings on the impact of PsJN on phenolic metabolism allowed us to identify a supplementary biocontrol mechanism developed by this PGPR to induce plant resistance against pathogens.

摘要

酚类化合物与植物-微生物相互作用有关，可能是植物促生根际细菌（PGPR）诱导产生的。在 PGPR 中，有益细菌 PsJN 先前被描述为能刺激植物生长，并能更好地适应非生物和生物胁迫。本研究旨在研究 PsJN 对葡萄次生代谢的影响。为此，比较了非细菌化和 PsJN 细菌化的葡萄苗的根和叶的基因表达（qRT-PCR）和次生代谢物（UHPLC-UV/DAD-MS QTOF）的分析。我们的结果表明，PsJN 在局部（根）和系统（叶）诱导 和 的过表达，特别是在叶中诱导苯丙烷和类黄酮途径的所有基因的过表达。此外，代谢组学方法表明，根和叶中分别有 32 种和 17 种化合物的相对含量因 PsJN 而显著改变。通过代谢组学方法确定为响应 PsJN 而积累的化合物，其对 孢子萌发的抑菌特性在体外进行了验证。总之，我们关于 PsJN 对酚类代谢影响的研究结果，使我们能够确定这种 PGPR 开发的一种补充生物防治机制，以诱导植物对病原体的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e352/6888286/915ea16426f0/ijms-20-05775-g010.jpg
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