Chahota Rajesh, Ogawa Hirohito, Mitsuhashi Yoko, Ohya Kenji, Yamaguchi Tsuyoshi, Fukushi Hideto
Department of Applied Veterinary Science, United Graduate School of Veterinary Sciences, Gifu, Japan.
Microbiol Immunol. 2006;50(9):663-78. doi: 10.1111/j.1348-0421.2006.tb03839.x.
To study genetic diversity and occurrence of Chlamydophila psittaci, a total of 1,147 samples from 11 avian orders including 53 genera and 113 species of feral and captive birds were examined using ompA gene based nested PCR. Three types of chlamydiae: C. psittaci (94.12%), C. abortus (4.41%) and unknown Chlamydophila sp. (1.47%) were identified among 68 (5.93%) positive samples (Psittaciformes-59, Ciconiiformes-8 and Passeriformes-1). Based on nucleotide sequence variations in the VD2 region of ompA gene, all 64 detected C. psittaci strains were grouped into 4 genetic clusters. Clusters I, II, III and IV were detected from 57.35%, 19.12%, 10.29% and 7.35% samples respectively. A single strain of unknown Chlamydophila sp. was found phylogenetically intermediate between Chlamydophila species infecting avian and mammalian hosts. Among Psittaciformes, 28 out of 81 tested species including 10 species previously unreported were found to be chlamydiae positive. Chlamydiosis was detected among 8.97% sick and 48.39% dead birds as well 4.43% clinically normal birds. Therefore, it was observed that though various genetically diverse chlamydiae may cause avian chlamydiosis, only a few C. psittaci strains are highly prevalent and frequently associated with clinical/subclinical infections.
为研究鹦鹉热衣原体的遗传多样性及发生情况，使用基于ompA基因的巢式PCR对来自11个目、53属、113种野生和圈养鸟类的1147份样本进行了检测。在68份（5.93%）阳性样本（鹦形目-59份、鹳形目-8份、雀形目-1份）中鉴定出三种衣原体：鹦鹉热衣原体（94.12%）、流产衣原体（4.41%）和未知嗜衣原体种（1.47%）。根据ompA基因VD2区域的核苷酸序列变异，所有64株检测到的鹦鹉热衣原体菌株被分为4个遗传簇。分别从57.35%、19.12%、10.29%和7.35%的样本中检测到簇I、II、III和IV。发现一株未知嗜衣原体种在感染鸟类和哺乳动物宿主的嗜衣原体物种之间处于系统发育中间位置。在鹦形目中，81种受测物种中有28种呈衣原体阳性，其中包括10种先前未报告的物种。在8.97%的患病鸟类、48.39%的死亡鸟类以及4.43%临床正常的鸟类中检测到衣原体病。因此，据观察，尽管各种遗传多样性的衣原体可能导致禽衣原体病，但只有少数鹦鹉热衣原体菌株高度流行，并经常与临床/亚临床感染相关。
