Van Valkenburg C F, Marani E, Boon M E, Visser P
Department of Physiology, Leiden University, The Netherlands.
Histochem J. 1990 Jun-Jul;22(6-7):353-7. doi: 10.1007/BF01003169.
The fixation of the neurotransmitter dopamine in the central nervous system by perfusion with formalin solutions seems to take place mainly via the formalin-induced condensation product norsalsolinol. In the present investigation the influence of microwave irradiation of the formalin-induced condensation of dopamine was studied in vitro and in vivo by making use of different, relatively low, formalin concentrations. It appeared that in vitro and in vivo the dopamine conversion was complete with 4% formalin and no influence of microwaves was noted. However, by making use of much lower formalin concentrations (0.2% and 0.4%) the condensation of dopamine was strongly augmented, in vitro (200%) and in vivo (at least 500%) using microwave techniques. There was a considerable loss in non-microwaved tissue (30%) after perfusion in vivo. This was lower (10%) in microwaved tissue. In experiments with perfused brain tissue which allowed a more complete calculation, a loss was found. This might be caused by a strong binding of dopamine and/or norsalsolinol to tissue components or to side reactions that could not be traced by the present experimental techniques.
通过用福尔马林溶液灌注来固定中枢神经系统中的神经递质多巴胺，似乎主要是通过福尔马林诱导的缩合产物去甲猪毛菜酚来实现的。在本研究中，利用不同的、相对较低的福尔马林浓度，在体外和体内研究了微波辐射对福尔马林诱导的多巴胺缩合的影响。结果表明，在体外和体内，用4%福尔马林时多巴胺转化完成，未观察到微波的影响。然而，使用低得多的福尔马林浓度（0.2%和0.4%）时，采用微波技术，多巴胺的缩合在体外（增加200%）和体内（至少增加500%）都显著增强。体内灌注后，未微波处理的组织有相当大的损失（30%）。在微波处理的组织中损失较低（10%）。在对灌注脑组织进行的实验中，该实验允许进行更完整的计算，发现了损失。这可能是由于多巴胺和/或去甲猪毛菜酚与组织成分的强烈结合，或由于目前实验技术无法追踪的副反应所致。
