一种来自龙舌兰的新型甾体皂苷。

A new steroidal saponin from Agave attenuata.

作者信息

Mendes Tatiana Paz, Silva Graziela de Medeiros, da Silva Bernadete Pereira, Parente José Paz

机构信息

Laboratório de Química de Plantas Medicinais, Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, PO Box 68045 CEP, 21944-970 Rio de Janeiro, Brazil.

出版信息

Nat Prod Res. 2004 Apr;18(2):183-8. doi: 10.1080/14786410310001608127.

DOI:10.1080/14786410310001608127

PMID:14984094

Abstract

A new steroidal saponin was isolated from the leaves of Agave attenuata. Its structure was established as (3beta,beta,25S)-spirostan-3-yl O-beta-D-glucopyranosyl-(1 --> 2)-beta-D-glucopyranosyl-(1 --> 2)-O-[beta-D-glucopyranosyl-(1 --> 3)]-beta-D-glucopyranosyl-(1 --> 4)-beta-D-galactopyranoside. The structural identification was performed using detailed analyses of 1H- and 13C-NMR spectra including 2D NMR spectroscopic techniques (COSY, HETCOR, and COLOC) and chemical conversions. The hemolytic activity of the steroidal saponin was evaluated using an in vitro assay.

摘要

从龙舌兰的叶子中分离出一种新的甾体皂苷。其结构确定为(3β,β,25S)-螺甾烷-3-基 O-β-D-吡喃葡萄糖基-(1→2)-β-D-吡喃葡萄糖基-(1→2)-O-[β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖基-(1→4)-β-D-吡喃半乳糖苷。通过对1H-和13C-NMR光谱进行详细分析，包括二维NMR光谱技术（COSY、HETCOR和COLOC）以及化学转化来进行结构鉴定。使用体外试验评估甾体皂苷的溶血活性。