Sánchez J, Souriau A, Buendía A J, Arricau-Bouvery N, Martínez C M, Salinas J, Rodolakis A, Navarro J A
Departamento de Anatomía y Anatomía Patológica Comparadas.
Pathologie Infectieuse et Immunologie, INRA, Tours-Nouzilly, 37380 Nouzilly, France.
J Comp Pathol. 2006 Aug-Oct;135(2-3):108-115. doi: 10.1016/j.jcpa.2006.06.003.
Pregnant goats were inoculated subcutaneosly with Coxiella burnetii and the course of infection was studied. Abortion in the last third of pregnancy occurred in all infected animals. Tissues from the placenta and other organs were studied before and after abortion by immunohistochemistry and PCR analysis. After infection, mild lesions were observed in several maternal organs, mainly the mammary gland but also the lung and the liver. The trophoblast cells of the choriallantoic membrane were the first target cells of the placenta; there was, however, a substantial delay between initial infection and placental colonization. In the last weeks of pregnancy, just before abortion, massive bacterial multiplication was detected in the placenta. In this stage of infection a necrotic and suppurative placentitis separated the fetal trophoblast cells from maternal syncytial epithelium. Vasculitis was observed in the fetal mesenchyme. A strong maternal T-cell response was detected in the inter-placentomal areas but not in the placentomes, where only neutrophils and smaller numbers of macrophages were associated with the lesions. Neither lesions nor C. burnetii DNA were found in maternal organs in animals maintained until day 120 post-abortion.
将怀孕山羊皮下接种伯氏考克斯体，并对感染过程进行研究。所有感染动物在妊娠最后三分之一阶段均发生流产。在流产前后，通过免疫组织化学和聚合酶链反应（PCR）分析对胎盘和其他器官的组织进行研究。感染后，在几个母体器官中观察到轻度病变，主要是乳腺，还有肺和肝脏。绒毛膜尿囊膜的滋养层细胞是胎盘的首要靶细胞；然而，从最初感染到胎盘定植之间存在相当长的延迟。在妊娠最后几周，就在流产前，在胎盘中检测到大量细菌繁殖。在感染的这个阶段，坏死性和化脓性胎盘炎使胎儿滋养层细胞与母体合体上皮分离。在胎儿间充质中观察到血管炎。在胎盘小叶间区域检测到强烈的母体T细胞反应，但在胎盘小叶中未检测到，在胎盘小叶中只有中性粒细胞和少量巨噬细胞与病变有关。在流产后维持到第120天的动物的母体器官中未发现病变和伯氏考克斯体DNA。
