van Moll P, Baumgärtner W, Eskens U, Hänichen T
Institut für Veterinär-Pathologie, Justus-Liebig-Universität Giessen, Germany.
J Comp Pathol. 1993 Oct;109(3):295-301. doi: 10.1016/s0021-9975(08)80254-x.
Coxiella burnetii antigen was demonstrated immunocytochemically in the placental tissues of aborted fetuses from four cows and two ewes and from one full-term calf. Antigen was observed in cytoplasmic vacuoles of trophoblasts, especially along the base of the chorionic villi. Positive trophoblasts were of normal size or exhibited striking cytoplasmic enlargement. Antigen was also demonstrated in neutrophils, degenerated cells and extracellularly in the intervillous spaces. The chorioallantoic connective tissue, allantoic surface and fetal tissues were devoid of C. burnetii antigen. Ovine placentas showed strong coxiella-specific staining and histological lesions characterized by moderate to severe, multifocal, necrotizing and purulent inflammation. In bovine placentas, the number of immunopositive cells varied from few to moderate and changes were mild or absent. Placental vasculitis consisting of infiltrating mononuclear cells, neutrophils or eosinophils was observed in both of the ovine and two of the bovine fetuses. In addition, a few intravascular thrombi were observed in one ovine and one bovine placenta. Thus the ovine coxiellosis cases were associated with an abundant accumulation of C. burnetii antigen and with severe necrotizing purulent placentitis, whereas in bovine coxiellosis the lesions were mild or absent and the number of organisms demonstrated immunocytochemically was much smaller. These results suggest a relation between species, lesion severity and weight of infection.
通过免疫细胞化学方法在4头奶牛、2头母羊流产胎儿以及1头足月小牛的胎盘组织中检测到了伯氏考克斯体抗原。抗原见于滋养层细胞的胞质空泡中，尤其是沿绒毛膜绒毛基部。阳性滋养层细胞大小正常或呈现明显的胞质肿大。抗原也见于中性粒细胞、变性细胞以及绒毛间隙的细胞外。尿囊绒毛膜结缔组织、尿囊表面和胎儿组织未检测到伯氏考克斯体抗原。绵羊胎盘呈现强烈的考克斯体特异性染色以及以中度至重度、多灶性、坏死性和脓性炎症为特征的组织学病变。在牛胎盘中，免疫阳性细胞数量从少到中等不等，变化轻微或无变化。在绵羊和2头牛胎儿的胎盘中均观察到由浸润的单核细胞、中性粒细胞或嗜酸性粒细胞组成的胎盘血管炎。此外，在1个绵羊胎盘和1个牛胎盘中观察到少量血管内血栓形成。因此，绵羊考克斯体病病例与伯氏考克斯体抗原的大量积聚以及严重的坏死性脓性胎盘炎有关，而在牛考克斯体病中，病变轻微或无病变，免疫细胞化学检测到的病原体数量要少得多。这些结果提示了物种、病变严重程度和感染程度之间的关系。
