Jin Wei, Ge Ri-Li, Wei Quan-Jia, Bao Tian-You, Shi Hai-Ming, Tu Peng-Fei
School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, China.
J Chromatogr A. 2006 Nov 3;1132(1-2):320-4. doi: 10.1016/j.chroma.2006.08.022. Epub 2006 Sep 26.
A novel, accurate and valid fingerprint method was developed by using high-performance liquid chromatography-photodiode array detection (HPLC-DAD) to control the quality of Rheum tanguticum Maxim. ex Balf. By comprehensively comparing chromatograms of all samples, our result revealed that the chromatographic fingerprint we developed combining similarity evaluation could efficiently identify and distinguish raw materials of R. tanguticum from different sources. Besides, 21 characteristic peaks in chromatogram of R. tanguticum were unambiguously confirmed by comparison of reference compounds based on their retention time (t(R)) and UV spectra.
通过高效液相色谱 - 光电二极管阵列检测（HPLC - DAD）开发了一种新颖、准确且有效的指纹图谱方法，用于控制唐古特大黄（Rheum tanguticum Maxim. ex Balf.）的质量。通过全面比较所有样品的色谱图，我们的结果表明，我们开发的结合相似度评价的色谱指纹图谱能够有效地识别和区分不同来源的唐古特大黄原料。此外，通过基于保留时间（t(R)）和紫外光谱比较对照品，明确确认了唐古特大黄色谱图中的21个特征峰。
