Yang Xian, Yang Shui-Ping, Zhang Xue, Yu Xiao-Dong, He Qi-Yi, Wang Bo-Chu
College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China. ; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
College of Resources and Environment, Southwest University, Chongqing, 400716, P.R. China.
Iran J Pharm Res. 2014 Fall;13(4):1191-201.
The aim of this paper is to develop a rapid and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. і)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide Ⅰ, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ⅱ) The precision, stability and repeatability of fingerprinting were investigated. In addition, common peaks number, the percentage of non-common peaks and similarity were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable.
本文旨在以复方中药五味肠炎宁颗粒及其处方中的5味药材为研究对象，建立一种快速、高灵敏度的多指标定量HPLC指纹图谱方法。对多指标定量指纹图谱进行了研究。i)选取分别来源于5味药材的芦丁、没食子酸、绿原酸、白术内酯Ⅰ、茯苓酸和芹菜素6种成分作为定量成分，采用HPLC法测定11批颗粒及相应5味药材中它们的含量。ii)考察了指纹图谱的精密度、稳定性和重复性。此外，还研究了共有峰数、非共有峰百分比和相似度。其中，颗粒中的21个共有峰可从5味药材中找到峰源，其中来自牛耳枫的有10个峰，来自辣蓼的有9个峰，来自白术的有3个峰，来自茯苓的有3个峰，来自广藿香的有5个峰。结果表明，该指纹图谱鉴别方法可靠。
