Tackmann K, Mattis R, Conraths F J
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestr. 55, 16868 Wusterhausen, Germany.
J Vet Med B Infect Dis Vet Public Health. 2006 Oct;53(8):395-8. doi: 10.1111/j.1439-0450.2006.01003.x.
The intestinal scraping technique (IST) is widely used for the detection of definitive hosts infected with Echinococcus multilocularis. The sensitivity of the method has been questioned in recent years. Several variations of the technique are used that may differ in their performance. We therefore estimated the diagnostic sensitivity and specificity of the IST protocol used in our lab by examining the small intestines of 210 E. multilocularis-infected foxes and 294 foxes that had tested negative for this parasite. To this end, IST was first performed on 48 and 294 intestines from infected and uninfected foxes respectively, followed by an examination of the entire remaining mucosa of the small intestine to discover any infections with E. multilocularis that had not been detected by IST. The point estimate for diagnostic sensitivity was 100%. Five different classes of infection intensities were formed. The lower limits of the 95% confidence intervals ranged between 76.8% and 92.6% depending on the number of samples analysed per class. When the small intestines of another 162 infected foxes were examined by IST and the results recorded separately for the anterior, middle and posterior third of the small intestine, a strong preference of E. multilocularis for the posterior third was observed. For epidemiological purposes, it may thus be possible to restrict routine investigations to the posterior portion of the small intestine. By contrast, a reduction of the number of slides examined per infected animal may lead to a considerable loss in sensitivity in animals with a low intensity of infection.
肠道刮片技术（IST）被广泛用于检测感染多房棘球绦虫的终末宿主。近年来，该方法的敏感性受到质疑。所使用的该技术的几种变体在性能上可能有所不同。因此，我们通过检查210只感染多房棘球绦虫的狐狸和294只该寄生虫检测呈阴性的狐狸的小肠，评估了我们实验室使用的IST方案的诊断敏感性和特异性。为此，首先分别对48只感染狐狸和294只未感染狐狸的肠道进行IST操作，然后检查小肠剩余的整个黏膜，以发现IST未检测到的多房棘球绦虫感染情况。诊断敏感性的点估计值为100%。形成了五种不同等级的感染强度。95%置信区间的下限根据每个等级分析的样本数量在76.8%至92.6%之间。当通过IST检查另外162只感染狐狸的小肠，并分别记录小肠前三分之一、中三分之一和后三分之一的结果时，观察到多房棘球绦虫强烈偏好于后三分之一。因此，出于流行病学目的，有可能将常规检查限制在小肠的后部。相比之下，减少每只感染动物检查的玻片数量可能会导致感染强度低的动物的敏感性大幅下降。
