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Miltefosine and antimonial drug susceptibility of Leishmania Viannia species and populations in regions of high transmission in Colombia.哥伦比亚高传播地区维氏利什曼原虫物种及群体对米替福新和锑剂的药物敏感性
PLoS Negl Trop Dis. 2014 May 22;8(5):e2871. doi: 10.1371/journal.pntd.0002871. eCollection 2014 May.
2
Comparison of conventional, molecular, and immunohistochemical methods in diagnosis of typical and atypical cutaneous leishmaniasis.比较传统、分子和免疫组化方法在典型和非典型皮肤利什曼病诊断中的应用。
Arch Pathol Lab Med. 2014 Feb;138(2):235-40. doi: 10.5858/arpa.2013-0098-OA.
3
Non-invasive cytology brush PCR for the diagnosis and causative species identification of American cutaneous leishmaniasis in Peru.秘鲁无创性细胞刷 PCR 检测用于美国皮肤利什曼病的诊断和致病种鉴定。
PLoS One. 2012;7(11):e49738. doi: 10.1371/journal.pone.0049738. Epub 2012 Nov 21.
4
Thermotherapy. An alternative for the treatment of American cutaneous leishmaniasis.热疗。一种治疗美国皮肤利什曼病的替代方法。
Trials. 2012 May 17;13:58. doi: 10.1186/1745-6215-13-58.
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Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: assessment of a molecular stepwise approach.秘鲁皮肤利什曼病患者临床样本中利什曼原虫（Viannia）种属鉴定：分子逐步分析方法评估。
J Clin Microbiol. 2012 Feb;50(2):495-8. doi: 10.1128/JCM.05061-11. Epub 2011 Nov 23.
6
Clinical pleiomorphism in human leishmaniases, with special mention of asymptomatic infection.人类利什曼病的临床多态性，特别提到无症状感染。
Clin Microbiol Infect. 2011 Oct;17(10):1451-61. doi: 10.1111/j.1469-0691.2011.03640.x.
7
Evaluation of 4 polymerase chain reaction protocols for cultured Leishmania spp. typing.评价 4 种聚合酶链反应方案在培养利什曼原虫种型鉴定中的应用。
Diagn Microbiol Infect Dis. 2010 Dec;68(4):401-9. doi: 10.1016/j.diagmicrobio.2010.08.007.
8
Clinical and demographic stratification of test performance: a pooled analysis of five laboratory diagnostic methods for American cutaneous leishmaniasis.临床和人口统计学的分层测试性能：五个实验室诊断方法对美国皮肤利什曼病的 pooled 分析。
Am J Trop Med Hyg. 2010 Aug;83(2):345-50. doi: 10.4269/ajtmh.2010.09-0414.
9
Heat-shock protein 70 PCR-RFLP: a universal simple tool for Leishmania species discrimination in the New and Old World.热休克蛋白 70 PCR-RFLP：新世界和旧世界利什曼原虫种属鉴别通用简单工具。
Parasitology. 2010 Jul;137(8):1159-68. doi: 10.1017/S0031182010000089. Epub 2010 May 5.
10
Detection and species identification of Leishmania DNA from filter paper lesion impressions for patients with American cutaneous leishmaniasis.从美国皮肤利什曼病患者滤纸皮损印迹中检测和鉴定利什曼原虫 DNA。
Clin Infect Dis. 2010 Jan 1;50(1):e1-6. doi: 10.1086/648730.

改进从皮肤病变的不同类型样本中鉴定利什曼原虫物种的方法。

Improving Leishmania species identification in different types of samples from cutaneous lesions.

作者信息

Cruz-Barrera Mónica L, Ovalle-Bracho Clemencia, Ortegon-Vergara Viviana, Pérez-Franco Jairo E, Echeverry Maria C

机构信息

Laboratorio de Parasitología, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.

Centro Dermatológico Federico Lleras Acosta, Bogotá, Colombia.

出版信息

J Clin Microbiol. 2015 Apr;53(4):1339-41. doi: 10.1128/JCM.02955-14. Epub 2015 Jan 21.

DOI:10.1128/JCM.02955-14
PMID:25609727
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4365241/
Abstract

The discrimination of Leishmania species from patient samples has epidemiological and clinical relevance. In this study, different gene target PCR-restriction fragment length polymorphism (RFLP) protocols were evaluated for their robustness as Leishmania species discriminators in 61 patients with cutaneous leishmaniasis. We modified the hsp70-PCR-RFLP protocol and found it to be the most reliable protocol for species identification.

摘要

从患者样本中鉴别利什曼原虫物种具有流行病学和临床意义。在本研究中，评估了不同基因靶点的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方案作为利什曼原虫物种鉴别方法在61例皮肤利什曼病患者中的稳健性。我们对热休克蛋白70（hsp70）-PCR-RFLP方案进行了改进，发现它是最可靠的物种鉴定方案。