改进从皮肤病变的不同类型样本中鉴定利什曼原虫物种的方法。
Improving Leishmania species identification in different types of samples from cutaneous lesions.
作者信息
Cruz-Barrera Mónica L, Ovalle-Bracho Clemencia, Ortegon-Vergara Viviana, Pérez-Franco Jairo E, Echeverry Maria C
机构信息
Laboratorio de Parasitología, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.
Centro Dermatológico Federico Lleras Acosta, Bogotá, Colombia.
出版信息
J Clin Microbiol. 2015 Apr;53(4):1339-41. doi: 10.1128/JCM.02955-14. Epub 2015 Jan 21.
Abstract
The discrimination of Leishmania species from patient samples has epidemiological and clinical relevance. In this study, different gene target PCR-restriction fragment length polymorphism (RFLP) protocols were evaluated for their robustness as Leishmania species discriminators in 61 patients with cutaneous leishmaniasis. We modified the hsp70-PCR-RFLP protocol and found it to be the most reliable protocol for species identification.
摘要
从患者样本中鉴别利什曼原虫物种具有流行病学和临床意义。在本研究中，评估了不同基因靶点的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方案作为利什曼原虫物种鉴别方法在61例皮肤利什曼病患者中的稳健性。我们对热休克蛋白70（hsp70）-PCR-RFLP方案进行了改进，发现它是最可靠的物种鉴定方案。
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