ILRAD, P.O. Box 30709, Nairobi, Kenya.
Plant Mol Biol. 1985 Jul;4(4):225-32. doi: 10.1007/BF02418240.
Unfractionated tRNA, isolated from maize mitochondria, has been specifically labeled at the -CCA end and used to recover a tRNA gene-bearing fragment from a clone bank of maize mitochondrial DNA. This gene has been mapped, sequenced and found to carry the anticodon for histidine. The sequence of the gene and that of bases in its near vicinity are identical to maize chloroplast tRNA(His), although sequences more distant on the fragment are not homologous with cpDNA. The junction of the cpDNA insert has been sequenced.
未分级 tRNA,从玉米线粒体中分离出来,已在 -CCA 末端进行了特异性标记,并用于从玉米线粒体 DNA 的克隆库中回收携带 tRNA 基因的片段。该基因已被定位、测序,并发现携带组氨酸的反密码子。该基因的序列及其附近碱基的序列与玉米叶绿体 tRNA(His)完全相同,尽管片段上更远的序列与 cpDNA 没有同源性。cpDNA 插入的连接已被测序。