Ainsworth S M, Nagy S B, Morgan L A, Miller G R, Perry J L
Department of Biological Sciences, Wichita State University, Kansas 67208.
J Clin Microbiol. 1990 Nov;28(11):2559-60. doi: 10.1128/jcm.28.11.2559-2560.1990.
Sputum specimens culture positive for Moraxella (Branhamella) catarrhalis were Gram stained with three decolorizer solutions (slow, 95% ethyl alcohol; intermediate, 1:1 ratio of 95% ethyl alcohol and acetone; and fast, acetone alone) for 5, 10, 20, and 30 s. Optimal results were obtained with acetone alone after 10 s or with a 1:1 mixture of acetone and ethanol after 20 s. Inadequate decolorization of M. catarrhalis in sputa is likely if the decolorization solution and exposure time are not optimal and may contribute to underreporting of this organism.
对卡他莫拉菌（布兰汉菌属）痰标本培养呈阳性的样本，用三种脱色剂溶液（慢速，95%乙醇；中速，95%乙醇与丙酮1:1比例混合；快速，仅用丙酮）进行革兰氏染色，染色时间分别为5秒、10秒、20秒和30秒。单独使用丙酮10秒或丙酮与乙醇1:1混合20秒可获得最佳结果。如果脱色剂溶液和暴露时间不理想，痰中卡他莫拉菌可能脱色不充分，这可能导致该菌报告不足。
