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使用RapID NH系统鉴定致病性奈瑟菌属菌种。

Identification of pathogenic Neisseria species with the RapID NH system.

作者信息

Robinson M J, Oberhofer T R

出版信息

J Clin Microbiol. 1983 Mar;17(3):400-4. doi: 10.1128/jcm.17.3.400-404.1983.

DOI:10.1128/jcm.17.3.400-404.1983
PMID:6404926
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272654/
Abstract

The RapID NH system (Innovative Diagnostics Systems, Inc., Decatur, Ga.) is a 4-h test used in the identification of Neisseria and Haemophilus species. The system was evaluated for accuracy and reliability and compared with conventional (cystine proteose peptone agar; Prepared Media Laboratory, Tualatin, Ore.) carbohydrate degradation tests with Neisseria gonorrhoeae and N. meningitidis, as well as a variety of Neisseria, Branhamella, and Moraxella species. The RapID NH system correctly identified all N. gonorrhoeae, N. meningitidis, and N. lactamica isolates, but the level of accuracy varied considerably for the remaining organisms. One strain of N. subflava was misidentified as a pathogenic Neisseria strain. The RapID NH tests were concluded in 4 h, whereas the cystine proteose peptone agar tests required up to 48 h for results to be useful. The RapID NH system is an accurate, reliable, and useful method for the identification of pathogenic Neisseria species. It has been proven that it shortens identification time and specimen turnaround time by at least 24 h.

摘要

RapID NH系统(创新诊断系统公司,佐治亚州迪凯特)是一种用于鉴定奈瑟菌属和嗜血杆菌属菌种的4小时检测方法。对该系统的准确性和可靠性进行了评估,并与传统的(胱氨酸蛋白胨琼脂;制备培养基实验室,俄勒冈州图拉丁)碳水化合物降解试验进行了比较,受试菌株包括淋病奈瑟菌、脑膜炎奈瑟菌,以及多种奈瑟菌属、布兰汉菌属和莫拉菌属菌种。RapID NH系统正确鉴定了所有淋病奈瑟菌、脑膜炎奈瑟菌和乳糖奈瑟菌分离株,但对于其余菌株,其准确程度差异很大。一株微黄奈瑟菌被误鉴定为致病性奈瑟菌菌株。RapID NH检测在4小时内完成,而胱氨酸蛋白胨琼脂试验需要长达48小时才能得出有用结果。RapID NH系统是一种准确、可靠且有用的致病性奈瑟菌属菌种鉴定方法。已证明它可将鉴定时间和标本周转时间至少缩短24小时。

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本文引用的文献

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Evaluation of the rapid penicillinase paper strip test for detection of beta-lactamase.用于检测β-内酰胺酶的快速青霉素酶纸条试验的评估
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