使用商用诺如病毒酶联免疫吸附测定试剂盒从重组病毒样颗粒和粪便样本中检测诺如病毒抗原。
Detection of norovirus antigens from recombinant virus-like particles and stool samples by a commercial norovirus enzyme-linked immunosorbent assay kit.
作者信息
Okitsu-Negishi Shoko, Okame Michio, Shimizu Yuko, Phan Tung Gia, Tomaru Takeshi, Kamijo Shigenori, Sato Takashi, Yagyu Fumihiro, Müller Werner E G, Ushijima Hiroshi
机构信息
Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan.
出版信息
J Clin Microbiol. 2006 Oct;44(10):3784-6. doi: 10.1128/JCM.01373-06.
Abstract
The commercial norovirus enzyme-linked immunosorbent assay kit was evaluated for its reactivity to recombinant virus-like particles and the detection of natural viruses from stool samples of Japanese infants and children with sporadic acute gastroenteritis compared to reverse transcription-PCR. The kit had a sensitivity of 76.3% and a specificity of 94.9%. Our results clearly indicated that the kit allows the detection of the most prevalent genotype, GII/4. In order to increase the sensitivity of the kit, the reactivity with norovirus of GII/3 and GII/6 genotypes needs to be improved.
摘要
对商用诺如病毒酶联免疫吸附测定试剂盒进行了评估，检测其对重组病毒样颗粒的反应性，并与逆转录聚合酶链反应相比，检测日本散发性急性胃肠炎婴幼儿粪便样本中的天然病毒。该试剂盒的灵敏度为76.3%，特异性为94.9%。我们的结果清楚地表明，该试剂盒能够检测出最常见的基因型GII/4。为了提高该试剂盒的灵敏度，需要提高其对GII/3和GII/6基因型诺如病毒的反应性。
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