Okitsu-Negishi Shoko, Okame Michio, Shimizu Yuko, Phan Tung Gia, Tomaru Takeshi, Kamijo Shigenori, Sato Takashi, Yagyu Fumihiro, Müller Werner E G, Ushijima Hiroshi
Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan.
J Clin Microbiol. 2006 Oct;44(10):3784-6. doi: 10.1128/JCM.01373-06.
The commercial norovirus enzyme-linked immunosorbent assay kit was evaluated for its reactivity to recombinant virus-like particles and the detection of natural viruses from stool samples of Japanese infants and children with sporadic acute gastroenteritis compared to reverse transcription-PCR. The kit had a sensitivity of 76.3% and a specificity of 94.9%. Our results clearly indicated that the kit allows the detection of the most prevalent genotype, GII/4. In order to increase the sensitivity of the kit, the reactivity with norovirus of GII/3 and GII/6 genotypes needs to be improved.
对商用诺如病毒酶联免疫吸附测定试剂盒进行了评估,检测其对重组病毒样颗粒的反应性,并与逆转录聚合酶链反应相比,检测日本散发性急性胃肠炎婴幼儿粪便样本中的天然病毒。该试剂盒的灵敏度为76.3%,特异性为94.9%。我们的结果清楚地表明,该试剂盒能够检测出最常见的基因型GII/4。为了提高该试剂盒的灵敏度,需要提高其对GII/3和GII/6基因型诺如病毒的反应性。