• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Conservative inheritance of newly synthesized DNA in double-strand break-induced gene conversion.双链断裂诱导的基因转换中新合成DNA的保守遗传
Mol Cell Biol. 2006 Dec;26(24):9424-9. doi: 10.1128/MCB.01654-06. Epub 2006 Oct 9.
2
Role of Double-Strand Break End-Tethering during Gene Conversion in Saccharomyces cerevisiae.双链断裂末端系留作用在酿酒酵母基因转换过程中的角色
PLoS Genet. 2016 Apr 13;12(4):e1005976. doi: 10.1371/journal.pgen.1005976. eCollection 2016 Apr.
3
Removal of nonhomologous DNA ends in double-strand break recombination: the role of the yeast ultraviolet repair gene RAD1.双链断裂重组中非同源DNA末端的去除：酵母紫外线修复基因RAD1的作用。
Science. 1992 Oct 16;258(5081):480-4. doi: 10.1126/science.1411547.
4
A single Ho-induced double-strand break at the MAT locus is lethal in Candida glabrata.在光滑球拟酵母中，MAT 基因座处单个 Ho 诱导的双链断裂是致命的。
PLoS Genet. 2020 Oct 15;16(10):e1008627. doi: 10.1371/journal.pgen.1008627. eCollection 2020 Oct.
5
Two different types of double-strand breaks in Saccharomyces cerevisiae are repaired by similar RAD52-independent, nonhomologous recombination events.酿酒酵母中两种不同类型的双链断裂通过类似的不依赖RAD52的非同源重组事件进行修复。
Mol Cell Biol. 1994 Feb;14(2):1293-301. doi: 10.1128/mcb.14.2.1293-1301.1994.
6
Conservative repair of a chromosomal double-strand break by single-strand DNA through two steps of annealing.通过单链DNA经两步退火对染色体双链断裂进行保守修复。
Mol Cell Biol. 2006 Oct;26(20):7645-57. doi: 10.1128/MCB.00672-06. Epub 2006 Aug 14.
7
A recombination execution checkpoint regulates the choice of homologous recombination pathway during DNA double-strand break repair.重组执行检查点在DNA双链断裂修复过程中调节同源重组途径的选择。
Genes Dev. 2009 Feb 1;23(3):291-303. doi: 10.1101/gad.1751209.
8
Monitoring DNA recombination initiated by HO endonuclease.监测由HO内切核酸酶引发的DNA重组。
Methods Mol Biol. 2012;920:349-70. doi: 10.1007/978-1-61779-998-3_25.
9
Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication.酵母中缺乏RAD51时的双链断裂修复：断裂诱导的DNA复制的可能作用。
Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7131-6. doi: 10.1073/pnas.93.14.7131.
10
Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis.通过 Southern 印迹分析监测芽殖酵母中的基因转换。
Methods Mol Biol. 2021;2153:221-238. doi: 10.1007/978-1-0716-0644-5_16.

引用本文的文献

1
Mutations and structural variants arising during double-strand break repair.双链断裂修复过程中出现的突变和结构变异。
bioRxiv. 2025 Mar 6:2025.02.28.640809. doi: 10.1101/2025.02.28.640809.
2
Double-strand break repair-associated intragenic deletions and tandem duplications suggest the architecture of the repair replication fork.双链断裂修复相关的基因内缺失和串联重复提示了修复复制叉的结构。
bioRxiv. 2023 Oct 13:2023.10.09.561461. doi: 10.1101/2023.10.09.561461.
3
What makes Komagataella phaffii non-conventional?是什么让糠秕假丝酵母具有非常规性？
FEMS Yeast Res. 2021 Dec 24;21(8). doi: 10.1093/femsyr/foab059.
4
Break-induced replication mechanisms in yeast and mammals.酵母和哺乳动物中的断裂诱导复制机制。
Curr Opin Genet Dev. 2021 Dec;71:163-170. doi: 10.1016/j.gde.2021.08.002. Epub 2021 Sep 1.
5
Mechanisms restraining break-induced replication at two-ended DNA double-strand breaks.抑制两端 DNA 双链断裂处引发复制的机制。
EMBO J. 2021 May 17;40(10):e104847. doi: 10.15252/embj.2020104847. Epub 2021 Apr 12.
6
Collaborations between chromatin and nuclear architecture to optimize DNA repair fidelity.染色质与核架构的协同作用以优化 DNA 修复保真度。
DNA Repair (Amst). 2021 Jan;97:103018. doi: 10.1016/j.dnarep.2020.103018. Epub 2020 Nov 22.
7
DNA Repair: The Search for Homology.DNA 修复：同源性的探索。
Bioessays. 2018 May;40(5):e1700229. doi: 10.1002/bies.201700229. Epub 2018 Mar 30.
8
Impact of Homologous Recombination on Silent Chromatin in .同源重组对沉默染色质的影响。
Genetics. 2018 Mar;208(3):1099-1113. doi: 10.1534/genetics.118.300704. Epub 2018 Jan 16.
9
Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing.靶向 DNA 断裂的修复：Cas9 介导的基因编辑的传统教学法。
ACS Chem Biol. 2018 Feb 16;13(2):397-405. doi: 10.1021/acschembio.7b00760. Epub 2017 Nov 14.
10
Position effects influencing intrachromosomal repair of a double-strand break in budding yeast.影响芽殖酵母中双链断裂的染色体内修复的位置效应
PLoS One. 2017 Jul 11;12(7):e0180994. doi: 10.1371/journal.pone.0180994. eCollection 2017.

本文引用的文献

1
Sgs1 regulates gene conversion tract lengths and crossovers independently of its helicase activity.Sgs1独立于其解旋酶活性调节基因转换片段长度和交叉。
Mol Cell Biol. 2006 Jun;26(11):4086-94. doi: 10.1128/MCB.00136-06.
2
THE REPLICATION OF DNA IN ESCHERICHIA COLI.大肠杆菌中DNA的复制
Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671-82. doi: 10.1073/pnas.44.7.671.
3
Molecular and cellular dissection of mating-type switching steps in Schizosaccharomyces pombe.粟酒裂殖酵母交配型转换步骤的分子与细胞解析
Mol Cell Biol. 2005 Jan;25(1):303-11. doi: 10.1128/MCB.25.1.303-311.2005.
4
Recombination proteins in yeast.酵母中的重组蛋白。
Annu Rev Genet. 2004;38:233-71. doi: 10.1146/annurev.genet.38.072902.091500.
5
DNA end resection, homologous recombination and DNA damage checkpoint activation require CDK1.DNA末端切除、同源重组和DNA损伤检查点激活都需要CDK1。
Nature. 2004 Oct 21;431(7011):1011-7. doi: 10.1038/nature02964.
6
Formation, maintenance and consequences of the imprint at the mating-type locus in fission yeast.裂殖酵母交配型位点印记的形成、维持及后果。
EMBO J. 2004 Feb 25;23(4):930-8. doi: 10.1038/sj.emboj.7600099. Epub 2004 Feb 12.
7
The Bloom's syndrome helicase suppresses crossing over during homologous recombination.布卢姆氏综合征解旋酶在同源重组过程中抑制交叉。
Nature. 2003 Dec 18;426(6968):870-4. doi: 10.1038/nature02253.
8
Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast.Srs2和Sgs1-Top3在酵母双链断裂修复过程中抑制交叉。
Cell. 2003 Nov 14;115(4):401-11. doi: 10.1016/s0092-8674(03)00886-9.
9
Drosophila BLM in double-strand break repair by synthesis-dependent strand annealing.果蝇BLM在合成依赖链退火的双链断裂修复中发挥作用。
Science. 2003 Jan 10;299(5604):265-7. doi: 10.1126/science.1077198.
10
Extensive loss of heterozygosity is suppressed during homologous repair of chromosomal breaks.在染色体断裂的同源修复过程中，杂合性的广泛丧失受到抑制。
Mol Cell Biol. 2003 Jan;23(2):733-43. doi: 10.1128/MCB.23.2.733-743.2003.

双链断裂诱导的基因转换中新合成DNA的保守遗传

Conservative inheritance of newly synthesized DNA in double-strand break-induced gene conversion.

作者信息

Ira Grzegorz, Satory Dominik, Haber James E

机构信息

Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02453-2728, USA.

出版信息

Mol Cell Biol. 2006 Dec;26(24):9424-9. doi: 10.1128/MCB.01654-06. Epub 2006 Oct 9.

DOI:10.1128/MCB.01654-06
PMID:17030630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1698534/
Abstract

To distinguish among possible mechanisms of repair of a double-strand break (DSB) by gene conversion in budding yeast, Saccharomyces cerevisiae, we employed isotope density transfer to analyze budding yeast mating type (MAT) gene switching in G2/M-arrested cells. Both of the newly synthesized DNA strands created during gene conversion are found at the repaired locus, leaving the donor unchanged. These results support suggestions that mitotic DSBs are primarily repaired by a synthesis-dependent strand-annealing mechanism. We also show that the proportion of crossing-over associated with DSB-induced ectopic recombination is not affected by the presence of nonhomologous sequences at one or both ends of the DSB or the presence of additional sequences that must be copied from the donor.

摘要

为了区分出芽酵母（酿酒酵母）中通过基因转换修复双链断裂（DSB）的可能机制，我们采用同位素密度转移法来分析处于G2/M期阻滞的细胞中的芽殖酵母交配型（MAT）基因转换。在基因转换过程中产生的两条新合成的DNA链都出现在修复位点，而供体保持不变。这些结果支持了有丝分裂DSB主要通过合成依赖的链退火机制进行修复的观点。我们还表明，与DSB诱导的异位重组相关的交叉比例不受DSB一端或两端非同源序列的存在或必须从供体复制的其他序列的存在的影响。