双链断裂诱导的基因转换中新合成DNA的保守遗传
Conservative inheritance of newly synthesized DNA in double-strand break-induced gene conversion.
作者信息
Ira Grzegorz, Satory Dominik, Haber James E
机构信息
Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02453-2728, USA.
出版信息
Mol Cell Biol. 2006 Dec;26(24):9424-9. doi: 10.1128/MCB.01654-06. Epub 2006 Oct 9.
Abstract
To distinguish among possible mechanisms of repair of a double-strand break (DSB) by gene conversion in budding yeast, Saccharomyces cerevisiae, we employed isotope density transfer to analyze budding yeast mating type (MAT) gene switching in G2/M-arrested cells. Both of the newly synthesized DNA strands created during gene conversion are found at the repaired locus, leaving the donor unchanged. These results support suggestions that mitotic DSBs are primarily repaired by a synthesis-dependent strand-annealing mechanism. We also show that the proportion of crossing-over associated with DSB-induced ectopic recombination is not affected by the presence of nonhomologous sequences at one or both ends of the DSB or the presence of additional sequences that must be copied from the donor.
摘要
为了区分出芽酵母（酿酒酵母）中通过基因转换修复双链断裂（DSB）的可能机制，我们采用同位素密度转移法来分析处于G2/M期阻滞的细胞中的芽殖酵母交配型（MAT）基因转换。在基因转换过程中产生的两条新合成的DNA链都出现在修复位点，而供体保持不变。这些结果支持了有丝分裂DSB主要通过合成依赖的链退火机制进行修复的观点。我们还表明，与DSB诱导的异位重组相关的交叉比例不受DSB一端或两端非同源序列的存在或必须从供体复制的其他序列的存在的影响。
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本文引用的文献
1
Sgs1 regulates gene conversion tract lengths and crossovers independently of its helicase activity.Sgs1独立于其解旋酶活性调节基因转换片段长度和交叉。
Mol Cell Biol. 2006 Jun;26(11):4086-94. doi: 10.1128/MCB.00136-06.
2
THE REPLICATION OF DNA IN ESCHERICHIA COLI.大肠杆菌中DNA的复制
Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671-82. doi: 10.1073/pnas.44.7.671.
3
Molecular and cellular dissection of mating-type switching steps in Schizosaccharomyces pombe.粟酒裂殖酵母交配型转换步骤的分子与细胞解析
Mol Cell Biol. 2005 Jan;25(1):303-11. doi: 10.1128/MCB.25.1.303-311.2005.
4
Recombination proteins in yeast.酵母中的重组蛋白。
Annu Rev Genet. 2004;38:233-71. doi: 10.1146/annurev.genet.38.072902.091500.
5
DNA end resection, homologous recombination and DNA damage checkpoint activation require CDK1.DNA末端切除、同源重组和DNA损伤检查点激活都需要CDK1。
Nature. 2004 Oct 21;431(7011):1011-7. doi: 10.1038/nature02964.
6
Formation, maintenance and consequences of the imprint at the mating-type locus in fission yeast.裂殖酵母交配型位点印记的形成、维持及后果。
EMBO J. 2004 Feb 25;23(4):930-8. doi: 10.1038/sj.emboj.7600099. Epub 2004 Feb 12.
7
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8
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9
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