Kondo Minako, Nappa Jérôme, Ronayne Kate L, Stelling Allison L, Tonge Peter J, Meech Stephen R
J Phys Chem B. 2006 Oct 19;110(41):20107-10. doi: 10.1021/jp0650735.
Ultrafast time-resolved infrared (TRIR) spectra of flavin adenine dinucleotide (FAD) and the anion of lumiflavin (Lf-) are described. Ground-state recovery and excited-state decay of FAD reveal a common dominant ultrafast relaxation and a minor slower component. The Lf- transient lacks a fast component. No intermediate species are observed, suggesting that the quenching mechanism is internal conversion promoted by interaction of the adenine and isoalloxazine rings in FAD. Modes are assigned, and the potential for extension of the TRIR method to photoactive proteins is discussed.
描述了黄素腺嘌呤二核苷酸（FAD）和黄素的阴离子（Lf-）的超快时间分辨红外（TRIR）光谱。FAD的基态恢复和激发态衰减显示出一个共同的主要超快弛豫和一个较小的较慢成分。Lf-瞬态缺乏快速成分。未观察到中间物种，这表明猝灭机制是由FAD中腺嘌呤和异咯嗪环的相互作用促进的内转换。对模式进行了归属，并讨论了将TRIR方法扩展到光活性蛋白质的潜力。
