Aquino Victor H, Anatriello Elen, Gonçalves Paula F, DA Silva Eliana V, Vasconcelos Pedro F C, Vieira Deusylene S, Batista Weber C, Bobadilla Maria L, Vazquez Cynthia, Moran Mirian, Figueiredo Luiz T M
Laboratório de Virologia, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto, Brazil.
Am J Trop Med Hyg. 2006 Oct;75(4):710-5.
We studied the molecular epidemiology of dengue virus type 3 (DENV-3) in Brazil and Paraguay by analyzing the 5' and 3' untranslated regions (5' and 3'UTRs) and the E protein gene of viruses isolated between 2002 and 2004. Both 5' and 3'UTRs were highly conserved. However, the 3'UTR of two isolates from Brazil contained eight nucleotide deletions compared with the remaining 26 viruses. Phylogenetic analyses suggested that DENV-3 was introduced into Brazil from the Caribbean Islands at least twice and into Paraguay from Brazil at least three times.
我们通过分析2002年至2004年间分离出的病毒的5'和3'非翻译区（5'和3'UTRs）以及E蛋白基因，研究了巴西和巴拉圭登革热病毒3型（DENV-3）的分子流行病学。5'和3'UTRs均高度保守。然而，与其余26种病毒相比，来自巴西的两个分离株的3'UTR包含8个核苷酸缺失。系统发育分析表明，DENV-3至少两次从加勒比群岛传入巴西，至少三次从巴西传入巴拉圭。
