Lo Giudice A, Michaud L, de Pascale D, De Domenico M, di Prisco G, Fani R, Bruni V
Dipartimento di Biologia Animale ed Ecologia Marina, Università di Messina, Messina, Italy.
J Appl Microbiol. 2006 Nov;101(5):1039-48. doi: 10.1111/j.1365-2672.2006.03006.x.
The aim of this study was to investigate the lipolytic activity of cold-adapted Antarctic marine bacteria and, furthermore, the combined effect of some environmental factors on this enzymatic process.
Strains were assayed for lipolytic activity on a basal medium amended with seven individual fatty acid esters. A significant activity was observed for 148 isolates (95.5% of the total screened). The interactive effect of pH, temperature and NaCl concentration on the substrates was tested for six representative isolates, identified as Pseudoalteromonas, Psychrobacter and Vibrio. Differences between strains according to NaCl and pH tolerances were observed. Only one strain degraded the substrate more efficiently at 4 degrees C than at 15 degrees C.
Our findings demonstrate that the lipolytic activity of Antarctic marine bacteria is rather variable, depending on culture conditions, and occurs in a wide range of salt concentration and pH.
Isolation and characterization of bacteria that are able to efficiently remove lipids at low temperatures will provide insight into the possibility to use cold-adapted bacteria as a source of exploitable enzymes. Moreover, research on the interactive effects of salt concentration, pH and temperature will be useful to understand the true enzyme potentialities for industrial applications.
本研究旨在调查适应寒冷的南极海洋细菌的脂解活性，此外，还要研究一些环境因素对这一酶促过程的综合影响。
在添加了七种单一脂肪酸酯的基础培养基上测定菌株的脂解活性。观察到148株菌株（占筛选总数的95.5%）具有显著活性。对鉴定为假交替单胞菌属、嗜冷杆菌属和弧菌属的六株代表性菌株测试了pH、温度和NaCl浓度对底物的交互作用。观察到不同菌株在耐NaCl和pH方面存在差异。只有一株菌株在4℃时比在15℃时更有效地降解底物。
我们的研究结果表明，南极海洋细菌的脂解活性变化很大，这取决于培养条件，并且在广泛的盐浓度和pH范围内都有发生。
分离和鉴定能够在低温下有效去除脂质的细菌，将有助于深入了解利用适应寒冷的细菌作为可开发酶源的可能性。此外，研究盐浓度、pH和温度的交互作用将有助于了解这些酶在工业应用中的真正潜力。
