Khanna Sunali S, Karjodkar Freny R
Department of Oral Medicine and Radiology, Nair Hospital Dental College, Mumbai, India.
Head Face Med. 2006 Oct 16;2:33. doi: 10.1186/1746-160X-2-33.
To evaluate the levels of circulating immune complexes, trace elements (copper, iron and selenium) in serum of patients with oral submucous fibrosis (OSMF), oral leukoplakia (L), and oral squamous cell carcinoma (SCC), analyze the alteration and identify the best predictors amongst these parameters for disease occurrence and progression.
Circulating immune complexes (CIC) were estimated using 37.5% Polyethylene Glycol 6000(PEG) serum precipitation. Serum estimation of copper (Cu), Iron (Fe) and selenium (Se) was done using the Oxalyl Dihydrazide method, Colorimetric Dipyridyl method and the Differential Pulse Cathodic Stripping Voltametry respectively.
The data analysis revealed increased circulating immune complex levels in the precancer and cancer patients. Serum copper levels showed gradual increase from precancer to cancer patients. However, serum iron levels were decreased significantly in the cancer group. Selenium levels showed marked decrease in the cancer group. Among CIC, serum, copper, iron and selenium the best predictors for the occurrence of lesions were age, serum iron, CIC, serum selenium in the decreasing order.
The present study shows that these immunological and biological markers may be associated with the pathogenesis of oral premalignant and malignant lesions and their progressions. Concerted efforts would, therefore, help in early detection, management, and monitoring the efficacy of treatment.
评估口腔黏膜下纤维化（OSMF）、口腔白斑（L）及口腔鳞状细胞癌（SCC）患者血清中循环免疫复合物及微量元素（铜、铁和硒）的水平，分析其变化情况，并确定这些参数中疾病发生和进展的最佳预测指标。
采用37.5%聚乙二醇6000（PEG）血清沉淀法估算循环免疫复合物（CIC）。分别采用草酰二肼法、比色联吡啶法和微分脉冲阴极溶出伏安法测定血清铜（Cu）、铁（Fe）和硒（Se）。
数据分析显示，癌前和癌症患者的循环免疫复合物水平升高。血清铜水平从癌前患者到癌症患者呈逐渐升高趋势。然而，癌症组血清铁水平显著降低。癌症组硒水平显著降低。在CIC、血清、铜、铁和硒中，病变发生的最佳预测指标依次为年龄、血清铁、CIC、血清硒。
本研究表明，这些免疫和生物学标志物可能与口腔癌前和恶性病变的发病机制及其进展有关。因此，共同努力将有助于早期发现、管理和监测治疗效果。
