Major Hilary J, Williams Rebecca, Wilson Amy J, Wilson Ian D
Waters Corporation, Atlas Park, Simonsway, Manchester M22 5PP, UK.
Rapid Commun Mass Spectrom. 2006;20(22):3295-302. doi: 10.1002/rcm.2732.
Plasma obtained from three strains of Zucker rats was analysed using capillary gas chromatography/mass spectrometry (GC/MS) to obtain global metabolite profiles as part of a series of metabonomic investigations of animal models of diabetes. Samples were obtained from 20-week-old male wild-type Zucker lean, (fa/fa) obese and lean/(fa) animals and were analysed following protein precipitation, using acetonitrile, and derivatisation. Subsequent data analysis using principal components analysis (PCA) and orthogonal projection to latent structures (OPLS) revealed differences between the plasma metabolite profiles of the three strains, with those of the Zucker lean and the lean/(fa) crosses being similar to each other whilst differing from the (fa/fa) obese strain.
作为对糖尿病动物模型进行的一系列代谢组学研究的一部分，使用毛细管气相色谱/质谱联用仪（GC/MS）对从三株Zucker大鼠获得的血浆进行分析，以获取整体代谢物谱。样本取自20周龄的雄性野生型Zucker瘦鼠、（fa/fa）肥胖鼠和瘦/（fa）鼠，并在使用乙腈进行蛋白质沉淀和衍生化后进行分析。随后使用主成分分析（PCA）和潜在结构正交投影（OPLS）进行数据分析，结果显示这三株大鼠的血浆代谢物谱存在差异，其中Zucker瘦鼠和瘦/（fa）杂交鼠的代谢物谱彼此相似，但与（fa/fa）肥胖鼠不同。
