Williams R, Lenz E M, Wilson A J, Granger J, Wilson I D, Major H, Stumpf C, Plumb R
Department of Drug Metabolism and Pharmacokinetics, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire, UKSK10 4TG.
Mol Biosyst. 2006 Mar;2(3-4):174-83. doi: 10.1039/b516356k. Epub 2006 Jan 30.
Plasma obtained from 20 week old normal Wistar-derived and Zucker (fa/fa) rats was analysed using a number of different analytical methodologies to obtain global metabolite profiles as part of metabonomic investigations of animal models of diabetes. Samples were analysed without sample pre-treatment using 1H NMR spectroscopy, after acetonitrile solvent protein precipitation by ultra-performance liquid chromatography-MS (UPLC-MS) and after acetonitrile protein precipitation and derivatisation for capillary gas chromatography-MS (GC-MS). Subsequent data analysis using principal components analysis revealed that all three analytical platforms readily detected differences between the plasma metabolite profiles of the two strains of rat. There was only limited overlap between the metabolites detected by the different methodologies and the combination of all three methods of metabolite profiling therefore provided a much more comprehensive profile than would have been provided by their use individually.
从20周龄正常Wistar品系大鼠和Zucker（fa/fa）大鼠获得的血浆，使用多种不同的分析方法进行分析，以获得整体代谢物谱，作为糖尿病动物模型代谢组学研究的一部分。样品在未经样品预处理的情况下，使用1H NMR光谱进行分析；在通过超高效液相色谱 - 质谱联用仪（UPLC-MS）进行乙腈溶剂蛋白沉淀后进行分析；以及在乙腈蛋白沉淀和衍生化后用于毛细管气相色谱 - 质谱联用仪（GC-MS）进行分析。随后使用主成分分析进行数据分析，结果显示所有三个分析平台都能轻松检测到这两种品系大鼠血浆代谢物谱之间的差异。不同方法检测到的代谢物之间只有有限的重叠，因此这三种代谢物分析方法的组合提供了比单独使用它们更全面的谱图。
