Adachi A, Ono N, Sakai H, Ogawa K, Shibata R, Kiyomasu T, Masuike H, Ueda S
Institute for Virus Research, Kyoto University, Japan.
Arch Virol. 1991;117(1-2):45-58. doi: 10.1007/BF01310491.
Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations in gag (p17, p24, and p15 regions), pol (protease, reverse transcriptase, and endonuclease domains), env (gp120 region), tat, or rev were infectious, whereas vif, vpr, vpu, some of env (gp41) and nef mutants could grow in human CD4+ cells to various degrees. Of the non-infectious mutants, only endonuclease (pol) and gp41 mutants exhibited normal phenotypes with respect to the production of functional reverse transcriptase, the expression of gag, pol, and env proteins, and the generation of progeny virions, when examined in transient assays. All infectious mutants killed the CD4+ cells with the exception of a mutant carrying a defect in the vif gene.
利用重组DNA技术将突变引入1型人类免疫缺陷病毒感染性分子克隆的9个基因中。通过转染和感染实验对产生的24个突变体进行了生化和生物学特性分析。在gag（p17、p24和p15区域）、pol（蛋白酶、逆转录酶和核酸内切酶结构域）、env（gp120区域）、tat或rev中发生突变的突变体均无感染性，而vif、vpr、vpu、部分env（gp41）和nef突变体可在人CD4+细胞中不同程度地生长。在非感染性突变体中，在瞬时分析中检测时，只有核酸内切酶（pol）和gp41突变体在功能性逆转录酶的产生、gag、pol和env蛋白的表达以及子代病毒体的产生方面表现出正常表型。除了一个vif基因存在缺陷的突变体外，所有感染性突变体均杀死了CD4+细胞。
