Massoumi Alamouti Sepideh, Kim Jae-Jin, Humble Leland M, Uzunovic Adnan, Breuil Colette
Department of Wood Science, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
Antonie Van Leeuwenhoek. 2007 Jan;91(1):19-34. doi: 10.1007/s10482-006-9092-8. Epub 2006 Oct 26.
A number of ophiostomatoid fungi were isolated from the spruce-infesting bark beetle, Ips perturbatus and its galleries collected from felled spruce trees and logs in northern BC and the Yukon Territory. Isolates were identified to species using morphological characteristics, nuclear ribosomal DNA and partial beta-tubulin gene sequences. Thirteen morphological and phylogenetic species were identified among the isolates. Leptographium fruticetum, Leptographium abietinum, Ophiostoma bicolor, Ophiostoma manitobense, O. piceaperdum, and eight undescribed species of the genus Ophiostoma and the anamorph genera Leptographium, Hyalorhinocladiella, Ambrosiella and Graphium. A number of these species, i.e. L. fruticetum, Hyalorhinocladiella sp. 2, O. bicolor and O. manitobense, were isolated repeatedly from I. perturbatus, while others, i.e. Graphium sp. 1 and O. piceaperdum, seemed to be sporadic associates. Among all the isolates, L. fruticetum had the highest relative dominance in this survey. A high frequency of occurrence of this species with the beetle may indicate a specific relationship between the two partners.
从侵扰云杉的树皮甲虫——北方云杉八齿小蠹及其虫道中分离出了多种长喙壳类真菌，这些树皮甲虫及其虫道采自不列颠哥伦比亚省北部和育空地区砍伐的云杉树和原木。利用形态特征、核糖体DNA和部分β-微管蛋白基因序列对分离菌株进行了种属鉴定。在这些分离菌株中鉴定出了13个形态和系统发育种。包括细枝长喙壳菌、冷杉长喙壳菌、双色长喙壳菌、马尼托巴长喙壳菌、云杉长喙壳菌，以及长喙壳属的8个未描述种和其无性型属的细链格孢属、透明隔孢属、安布罗西菌属和黏束孢属。其中一些种，即细枝长喙壳菌、透明隔孢属2号种、双色长喙壳菌和马尼托巴长喙壳菌，多次从北方云杉八齿小蠹中分离得到，而其他种，即黏束孢属1号种和云杉长喙壳菌，似乎是偶发伴生菌。在本次调查中，细枝长喙壳菌在所有分离菌株中的相对优势度最高。该物种与甲虫的高发生率可能表明这两个伙伴之间存在特定关系。
