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嗜硫副球菌新种,一种来自活性污泥的硫氧化菌。

Paracoccus sulfuroxidans sp. nov., a sulfur oxidizer from activated sludge.

作者信息

Liu Xing-Yu, Wang Bao-Jun, Jiang Cheng-Ying, Liu Shuang-Jiang

机构信息

Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Zhong-Guan-Cun, Haidian, Beijing 100080, P. R. China.

出版信息

Int J Syst Evol Microbiol. 2006 Nov;56(Pt 11):2693-2695. doi: 10.1099/ijs.0.64548-0.

Abstract

Bacterial strain LW36T was isolated from activated sludge of a wastewater-treatment bioreactor. Cells of strain LW36T were Gram-negative coccoids to short rods, 1.0-1.2x0.5 microm in size. Colonies were cream-coloured, smooth and circular. Strain LW36T was hetero-organotrophic and chemolithotrophic and was able to use reduced sulfur as an energy resource. Growth was observed at 25-36 degrees C and pH 5-10. The most abundant cellular fatty acid of strain LW36T was C18:1omega7c (64.2%). The sole respiratory quinone was ubiquinone-10. The G+C content of the genomic DNA was 61.3 mol% (Tm). 16S rRNA gene sequence analysis indicated that strain LW36T was phylogenetically related to members of the genus Paracoccus, with similarities ranging from 92.4 to 94.9%. Based on these results, it is concluded that strain LW36T represents a novel species of the genus Paracoccus, for which the name Paracoccus sulfuroxidans is proposed. The type strain is strain LW36T (=CGMCC 1.5364T=JCM 14013T).

摘要

细菌菌株LW36T是从废水处理生物反应器的活性污泥中分离得到的。LW36T菌株的细胞为革兰氏阴性球菌至短杆菌,大小为1.0 - 1.2×0.5微米。菌落呈奶油色,光滑且圆形。LW36T菌株是异养兼性化能无机营养型,能够利用还原态硫作为能源。在25 - 36℃和pH值5 - 10的条件下可观察到生长。LW36T菌株最丰富的细胞脂肪酸是C18:1ω7c(64.2%)。唯一的呼吸醌是泛醌-10。基因组DNA的G + C含量为61.3 mol%(熔解温度法)。16S rRNA基因序列分析表明,LW36T菌株在系统发育上与副球菌属的成员相关,相似度在92.4%至94.9%之间。基于这些结果,得出结论:LW36T菌株代表副球菌属的一个新物种,为此提出名为硫氧化副球菌的名称。模式菌株是LW36T菌株(=CGMCC 1.5364T =JCM 14013T)。

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