Baginski I, Chemin I, Bouffard P, Hantz O, Trepo C
Hepatitis, AIDS, and Human Retrovirus Research Unit, National Institute of Health and Medical Research (INSERM), Lyon, France.
J Infect Dis. 1991 May;163(5):996-1000. doi: 10.1093/infdis/163.5.996.
Polymerase chain reaction (PCR) with a reverse transcriptase step characterized a specific transcription activity in hepatitis B virus (HBV)-infected peripheral blood mononuclear cells (PBMC) in two patients (1 and 2) with chronic hepatitis positive for antibody to hepatitis B core antigen (HBc). Patient 1 was also coinfected with human hepatitis delta virus. A patient who cleared HBV replication after antiviral treatment with vidarabine served as negative control. HBV-specific RNA poly A sequences were detected by PCR in PBMC of patients 1 and 2 even without detectable HBV DNA (patient 2) as shown by dot blot and PCR assays. RNA sequences were found in both the nucleus and cytoplasm. The demonstration of HBV mRNA sequences within PBMC suggests the transcription of viral DNA, in agreement with the findings of HBV surface antigen in PBMC. The results in patient 1 demonstrated HBV mRNA sequences in leukocytes even without PCR-detectable HBV DNA sequences, likely due to ongoing hepatitis delta virus replication.
采用带有逆转录酶步骤的聚合酶链反应（PCR），对两名乙肝核心抗原（HBc）抗体阳性的慢性肝炎患者（患者1和患者2）感染乙型肝炎病毒（HBV）的外周血单个核细胞（PBMC）中的特异性转录活性进行了表征。患者1还合并感染了人类丁型肝炎病毒。一名接受阿糖腺苷抗病毒治疗后清除HBV复制的患者作为阴性对照。通过PCR在患者1和患者2的PBMC中检测到HBV特异性RNA多聚腺苷酸序列，即使如斑点印迹和PCR检测所示，患者2中未检测到HBV DNA。在细胞核和细胞质中均发现了RNA序列。PBMC中HBV mRNA序列的证实表明病毒DNA发生了转录，这与PBMC中HBV表面抗原的发现一致。患者1的结果表明，即使没有PCR可检测到的HBV DNA序列，白细胞中也存在HBV mRNA序列，这可能是由于丁型肝炎病毒正在进行复制。
