乙型肝炎病毒感染患者外周血单个核细胞中前S1蛋白的检测

Detection of pre-S1 proteins in peripheral blood mononuclear cells from patients with HBV infection.

作者信息

Zoulim F, Vitvitski L, Bouffard P, Pichoud C, Rougier P, Lamelin J P, Trépo C

机构信息

Hepatitis and AIDS Research Unit, INSERM U271, Lyon, France.

出版信息

J Hepatol. 1991 Mar;12(2):150-6. doi: 10.1016/0168-8278(91)90931-z.

DOI:10.1016/0168-8278(91)90931-z

PMID:1711065

Abstract

The presence of pre-S1 proteins in peripheral blood mononuclear cell (PBMC) samples from 115 patients with different forms of hepatitis B virus (HBV) infection was investigated by Western blot. Among 67 chronic HBsAg carriers, HBV antigens were detected in the PBMC in 80% for HBsAg, 27% for HBc/e Ag and 34% for pre-S1 proteins. The detection of pre-S1 proteins in PBMC was significantly associated with the presence of serum markers of HBV replication (HBV DNA and/or DNA polymerase). In the group of 48 consecutive patients negative for serum HBsAg, but positive for anti-HBc with or without anti-HBs, HBsAg and pre-S1 proteins could be detected in PBMC. This finding was more frequent among anti-HIV-positive patients (77 and 23% of the cases, respectively) than in the negative ones (23 and 4% of the cases, respectively). The detection of HBV DNA and polyadenylated RNA in some of the PBMC samples positive for HBV proteins suggests that these proteins may be expressed in PBMC, especially during intense HBV replication. In patients negative for serum HBsAg, PBMC may constitute a reservoir of HBV.

摘要

采用蛋白质印迹法研究了115例不同形式乙型肝炎病毒（HBV）感染患者外周血单个核细胞（PBMC）样本中前S1蛋白的存在情况。在67例慢性HBsAg携带者中，PBMC中检测到HBV抗原的比例分别为：HBsAg 80%、HBeAg 27%、前S1蛋白34%。PBMC中前S1蛋白的检测与HBV复制血清标志物（HBV DNA和/或DNA聚合酶）的存在显著相关。在48例血清HBsAg阴性但抗HBc阳性（有或无抗HBs）的连续患者组中，PBMC中可检测到HBsAg和前S1蛋白。这一发现在抗HIV阳性患者中（分别为77%和23%）比在阴性患者中（分别为23%和4%）更常见。在一些HBV蛋白阳性的PBMC样本中检测到HBV DNA和多聚腺苷酸化RNA，提示这些蛋白可能在PBMC中表达，尤其是在HBV强烈复制期间。在血清HBsAg阴性的患者中，PBMC可能是HBV的储存库。