Cova L, Mehrotra R, Wild C P, Chutimataewin S, Cao S F, Duflot A, Prave M, Yu S Z, Montesano R, Trepo C
INSERM U271, Lyon France.
Br J Cancer. 1994 Jan;69(1):104-9. doi: 10.1038/bjc.1994.16.
The oncogenicity of Duck hepatitis B virus (DHBV) is unclear since hepatocellular carcinomas (HCCs) have been reported only in domestic ducks in Qidong, an area of China where hepatitis B virus (HBV) and aflatoxin B1 (AFB1) are risk factors for liver cancer in man. In order to better define the association between DHBV infection, AFB1 and HCC we analysed a series of 16 duck liver samples collected from local farms in Qidong. HCC was found in eight and cirrhosis in one of these samples. Furthermore bile duct proliferation, characteristic of AFB1 exposure in ducks and other animal species, was found in these ducks. Integration of DHBV DNA into cellular DNA was observed in only one out of four DHBV positive HCCs, indicating that viral integration is not prerequisite for tumour development. In four remaining HCCs the polymerase chain reaction (PCR) failed to show any DHBV DNA suggesting that liver tumours do occur in polymerase chain reaction (PCR) failed to show any DHBV DNA suggesting that liver tumours do occur in these ducks in the absence of DHBV infection. In addition, AFB1-DNA adducts were detected by hplc-immunoassay in one such DHBV-negative tumour. In summary we demonstrate that risk factors other than DHBV, including AFB1 exposure, may be important in duck liver carcinogenesis in Qidong.
鸭乙型肝炎病毒（DHBV）的致癌性尚不清楚，因为仅在中国启东地区的家鸭中报道过肝细胞癌（HCC），该地区乙型肝炎病毒（HBV）和黄曲霉毒素B1（AFB1）是人类肝癌的危险因素。为了更好地确定DHBV感染、AFB1与HCC之间的关联，我们分析了从启东当地农场采集的一系列16份鸭肝样本。在其中8份样本中发现了HCC，1份样本中发现了肝硬化。此外，在这些鸭子中还发现了胆管增生，这是鸭子和其他动物物种接触AFB1的特征。在4例DHBV阳性HCC中，仅在1例中观察到DHBV DNA整合到细胞DNA中，这表明病毒整合不是肿瘤发生的先决条件。在其余4例HCC中，聚合酶链反应（PCR）未能显示任何DHBV DNA，这表明在没有DHBV感染的情况下，这些鸭子确实发生了肝肿瘤。此外，通过高效液相色谱-免疫分析法在1例此类DHBV阴性肿瘤中检测到了AFB1-DNA加合物。总之，我们证明除DHBV外的其他危险因素，包括接触AFB1，可能在启东鸭肝癌发生中起重要作用。
