Meechaisue Chidchanok, Dubin Robert, Supaphol Pitt, Hoven Voravee P, Kohn Joachim
Department of Materials Technology, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand.
J Biomater Sci Polym Ed. 2006;17(9):1039-56. doi: 10.1163/156856206778365988.
Desaminotyrosyl-tyrosine ethyl ester (DTE) and desaminotyrosyl-tyrosine (DT) were used as monomers in the synthesis of two tyrosine-derived polycarbonates: the slow degrading homopolymer poly(DTE carbonate) and the fast degrading co-polymer poly(DTE-co-20%DT carbonate). Ultrafine fibers of these polymers were successfully fabricated using an electrospinning process. The effects of some solution and process parameters (i.e., polymer concentration, electrostatic field strength and solvent system) on morphological appearance and diameters of the obtained fibers were investigated by scanning electron microscopy (SEM). Smooth fibers were obtained at high enough solution concentrations (i.e., 15 and 20% (w/v)). The average fiber diameter was found to increase with increasing polymer concentration and applied electrostatic field strength. The electrospinnability of poly(DTE-co-20%DT carbonate) in dichloromethane was enhanced when methanol was used as the co-solvent. In all of the conditions investigated, the average diameter of the obtained smooth fibers ranged between 1.9 and 5.8 microm. A qualitative assessment of an as-spun mat of poly(DTE carbonate) fibers as a tissue scaffolding material showed that three different cultured cell lines appeared to adhere and propagate well within the scaffold. For poly(DTE carbonate) exceptionally high cell densities could be achieved after 10 days of cell culture.
去氨基酪氨酸乙酯（DTE）和去氨基酪氨酸（DT）被用作单体，用于合成两种酪氨酸衍生的聚碳酸酯：降解缓慢的均聚物聚（DTE碳酸酯）和降解快速的共聚物聚（DTE-co-20%DT碳酸酯）。使用静电纺丝工艺成功制备了这些聚合物的超细纤维。通过扫描电子显微镜（SEM）研究了一些溶液和工艺参数（即聚合物浓度、静电场强度和溶剂体系）对所得纤维形态外观和直径的影响。在足够高的溶液浓度（即15%和20%（w/v））下可获得光滑的纤维。发现平均纤维直径随聚合物浓度和施加的静电场强度的增加而增大。当使用甲醇作为共溶剂时，聚（DTE-co-20%DT碳酸酯）在二氯甲烷中的可静电纺丝性增强。在所研究的所有条件下，所得光滑纤维的平均直径在1.9至5.8微米之间。对聚（DTE碳酸酯）纤维的静电纺丝垫作为组织支架材料进行的定性评估表明，三种不同的培养细胞系似乎在支架内粘附并良好增殖。对于聚（DTE碳酸酯），细胞培养10天后可实现异常高的细胞密度。
