Watanabe Junji, Akashi Mitsuru
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Biomacromolecules. 2006 Nov;7(11):3008-11. doi: 10.1021/bm060488h.
As a first step toward hydroxyapatite (HAp) formation in agarose hydrogels, we have tailored the internal chemistry using an electrophoresis approach. HAp was formed using aqueous solutions of calcium chloride and disodium hydrophosphate, which were set in a conventional agarose electrophoresis apparatus. Calcium and phosphate ions provided cations and anions, respectively, and were shown to migrate into the agarose hydrogel toward the corresponding electrode side. HAp was formed after colliding with each ion. The time needed to reach complete HAp formation was 30 min, and 130 ng of HAp was formed in 1 mg of agarose hydrogel when the equilibrium swelling state was reached. The electrophoresis approach accelerated the HAp formation, and the linear velocity of 1 mm/min was shown to be roughly 15 times larger than that of simple diffusion (0.06 mm/min).
作为在琼脂糖水凝胶中形成羟基磷灰石（HAp）的第一步，我们采用电泳方法对内部化学性质进行了调整。使用氯化钙水溶液和磷酸氢二钠在传统琼脂糖电泳装置中形成HAp。钙离子和磷酸根离子分别提供阳离子和阴离子，并显示向琼脂糖水凝胶中相应电极侧迁移。离子相互碰撞后形成HAp。达到完全形成HAp所需的时间为30分钟，当达到平衡溶胀状态时，在1毫克琼脂糖水凝胶中形成了130纳克HAp。电泳方法加速了HAp的形成，并且显示1毫米/分钟的线速度大约比简单扩散（0.06毫米/分钟）快15倍。
