Malavé Lara C, Gorriño M T, Campelo C, Lardelli P, Cisterna R
Microbiology and Immunology Department, University of Basque Country, Bilbao, Spain.
Eur J Clin Microbiol Infect Dis. 1994 Mar;13(3):267-71. doi: 10.1007/BF01974550.
The polymerase chain reaction (PCR) was used to analyze the presence of hepatitis B virus (HBV) DNA in serum and peripheral blood mononuclear cells (PBMCs) from 20 patients with AIDS with and without conventional HBV serological markers. DNA sequences of HBV were detected in PBMCs from 13 patients, nine of whom were positive for anti-HBc only and four of whom were also positive for anti-HBs. When PBMCs from patients were activated in culture with phytohemagglutinin, the presence of HBsAg could be detected in the culture supernatants from four of 13 patients with HBV DNA in their PBMCs; for two of the four, HBV DNA could also be detected in the culture supernatant after DNA amplification. It was observed that HBV DNA sequences found in PBMCs can be reactivated by mitogen stimulation in some HIV-1 infected patients.
采用聚合酶链反应（PCR）分析了20例合并或不合并传统乙肝血清学标志物的艾滋病患者血清及外周血单个核细胞（PBMC）中乙肝病毒（HBV）DNA的存在情况。在13例患者的PBMC中检测到了HBV的DNA序列，其中9例仅抗-HBc阳性，4例抗-HBs也呈阳性。当用植物血凝素在培养中激活患者的PBMC时，在13例PBMC中存在HBV DNA的患者中，有4例的培养上清液中可检测到HBsAg；在这4例中的2例中，DNA扩增后培养上清液中也可检测到HBV DNA。观察到在一些HIV-1感染患者中，PBMC中发现的HBV DNA序列可被丝裂原刺激重新激活。
