在南澳大利亚农村地区近期爆发的疫情期间，通过血清PCR诊断急性Q热。
Diagnosis of acute Q fever by PCR on sera during a recent outbreak in rural south Australia.
作者信息
Turra M, Chang G, Whybrow D, Higgins G, Qiao M
机构信息
Consultant Microbiologist/Virologist, Institute of Medical and Veterinary Science, Rundle Mall, Adelaide, South Australia 5000, Australia.
出版信息
Ann N Y Acad Sci. 2006 Oct;1078:566-9. doi: 10.1196/annals.1374.112.
Diagnosis of Q fever has largely been dependent upon serology, which may lead to delayed diagnosis as seroconversion can take weeks to develop. During a recent Q fever outbreak (27 patients) in rural South Australia, we compared the diagnostic rate of serology with two separate real-time PCRs, the 27kDa outer membrane protein and the insertion sequence. PCR was positive (on either or both PCR assays) in sera of 67% of the patients. Median time required for making serological diagnosis was 17 days, compared with 4 days by PCR. Q fever PCR is an effective tool in the diagnosis of acute Q fever infection.
Q热的诊断很大程度上依赖于血清学检测，而血清学检测可能导致诊断延迟，因为血清转化可能需要数周时间才能出现。在南澳大利亚农村最近一次Q热疫情（27例患者）期间，我们将血清学检测的诊断率与两种不同的实时聚合酶链反应（PCR）进行了比较，这两种PCR分别检测27kDa外膜蛋白和插入序列。67%的患者血清中PCR呈阳性（两种PCR检测中一种或两种呈阳性）。血清学诊断所需的中位时间为17天，而PCR诊断仅需4天。Q热PCR是诊断急性Q热感染的有效工具。
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