Stagno S, Reynolds D W, Tsiantos A, Fuccillo D A, Long W, Alford C A
J Infect Dis. 1975 Nov;132(5):568-77. doi: 10.1093/infdis/132.5.568.
Infants with congenitally (38) and natally (17) acquired cytomegalovirus infection were prospectively studied by means of virologic and multiple serologic assays. These infections were characterized by chronic viral excretion (measured in years). The quantity of virus excreted in the urine during early infancy was significantly greater in infants who acquired infection in utero, particularly amon those born with overt disease; thereafter, all three groups (congenital symptomatic, congenital asymptomatic, and natal) excreted similar amounts of virus. The patterns of antibody responses, particularly the fluorescent antibody response to the early antigen and the complement-fixing antibody response, further indicated that congenitally infected infants (especially symptomatic ones) bear a greater antigenic burden than do natally infected infants. From a diagnostic standpoint, the test for fluorescent antibody to the late antigen was the most sensitive assay, whereas the test for complement-fixing antibody proved to be the least useful, The indirect hemagglutination assay, although performed only in infants with natal infection, was only slightly less sensitive than the fluorescent antibody procedure; by the former technique, diagnostic rises were detected in all but one infant after the onset of viruria.
通过病毒学和多种血清学检测方法，对先天性（38例）和出生时获得性（17例）巨细胞病毒感染的婴儿进行了前瞻性研究。这些感染的特点是病毒长期排泄（以年计）。宫内感染的婴儿在婴儿早期尿液中排出的病毒量明显更多，尤其是那些出生时患有明显疾病的婴儿；此后，三组（先天性有症状、先天性无症状和出生时获得性）排出的病毒量相似。抗体反应模式，特别是对早期抗原的荧光抗体反应和补体结合抗体反应，进一步表明先天性感染的婴儿（尤其是有症状的婴儿）比出生时获得性感染的婴儿承受更大的抗原负担。从诊断角度来看，晚期抗原荧光抗体检测是最敏感的检测方法，而补体结合抗体检测被证明最无用。间接血凝试验虽然仅在出生时获得性感染的婴儿中进行，但敏感性仅略低于荧光抗体检测方法；通过前一种技术，除一名婴儿外，所有婴儿在病毒尿发作后均检测到诊断性升高。
