Griffiths P D, Stagno S, Reynolds D W, Alford C A
Arch Virol. 1978;58(2):111-8. doi: 10.1007/BF01315403.
18 women, of known seropositivity, have been followed for between 18 and 66 months (mean 50.2 months) for viral excretion and serological changes. CMV was isolated from 58/146 (39.7 per cent) cultures from various sites, mostly cervix. A total of 129 sera were obtained and each was titrated in the late antigen, early antigen and anti-complement immunofluorescence assays, as well as the complement fixation and microneutralisation tests. From 3 women virus was consistently re-isolated, from 3 others virus was never re-isolated and the remaining 12 women excreted virus intermittently during the period of study. No significant changes in antibody titres could be detected by any of the 5 serological assays in any woman during periods of viral excretion. This suggests that local reactivation of latent CMV infection is not associated with a demonstrable systemic antibody response. Inter-assay correlations were sought for each of the 10 possible combinations of pairs of tests. Significant correlations were found for most pairs of tests, with the notable exception fo those involving the early antigen test. This confirms that antibodies detected in this assay are distinct from those detected by the other 4 serological assays.
对18名已知血清反应阳性的女性进行了18至66个月（平均50.2个月）的随访，观察病毒排泄情况和血清学变化。在来自不同部位（主要是宫颈）的146份培养物中，有58份（39.7%）分离出巨细胞病毒（CMV）。共采集了129份血清样本，并分别在晚期抗原、早期抗原和抗补体免疫荧光试验以及补体结合试验和微量中和试验中进行滴定。在3名女性中持续重新分离出病毒，在另外3名女性中从未重新分离出病毒，其余12名女性在研究期间间歇性地排出病毒。在任何女性的病毒排泄期间，5种血清学检测方法均未检测到抗体滴度有显著变化。这表明潜伏性CMV感染的局部重新激活与可证实的全身抗体反应无关。对10种可能的两两检测组合中的每一种都进行了检测间相关性分析。大多数两两检测组合都发现了显著相关性，但涉及早期抗原检测的组合明显例外。这证实了该检测方法中检测到的抗体与其他4种血清学检测方法检测到的抗体不同。
