Gong Y-S, Chen J, Zhang Q-Z, Zhang J-T
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China.
J Asian Nat Prod Res. 2006 Oct-Nov;8(7):649-56. doi: 10.1080/10286020500246063.
To study the anti-osteoporosis effects and mechanism of action of oestradiol (E2) and ginsenoside (tR), we measured the bone mineral densities (BMD) of lumbar vertebra and tibia and analysed the tibia histological morphological data, as well observed the activity and the number of osteoblasts and the activity of alkaline phosphatase (ALP) and the concentration of cAMP. Results showed that E2 (400 microg kg- 1 week- 1) and tR (10, 20, 30 mg kg- 1 day- 1) were able to countervail the decreasing in BMDs of lumbar vertebra and tibia induced by OVX in rats (P<0.05); E2 (0.1 micromol l- 1) and ginsenoside Rg1 (1 micromol l- 1 and 10 micromol l- 1) were able to increase the number of osteoblasts, the activity of ALP and the concentration of intercellular cAMP in cultured osteoblast cells. The present findings suggest that E2 and tR have an anti-osteoporosis effect in ovariectomised rats.
为研究雌二醇(E2)和人参皂苷(tR)的抗骨质疏松作用及其作用机制,我们测量了大鼠腰椎和胫骨的骨密度(BMD),分析了胫骨组织形态学数据,并观察了成骨细胞的活性和数量、碱性磷酸酶(ALP)的活性以及环磷酸腺苷(cAMP)的浓度。结果显示,E2(400μg kg-1周-1)和tR(10、20、30mg kg-1天-1)能够对抗去卵巢大鼠腰椎和胫骨骨密度的降低(P<0.05);E2(0.1μmol l-1)和人参皂苷Rg1(1μmol l-1和10μmol l-1)能够增加培养的成骨细胞中的成骨细胞数量、ALP活性和细胞内cAMP浓度。目前的研究结果表明,E2和tR对去卵巢大鼠具有抗骨质疏松作用。