McKane A M, McMillan S A, Conlan S K, McNeill T A
Regional Immunology Laboratory, Belfast City Hospital.
Ir J Med Sci. 1991 Mar;160(3):93-5. doi: 10.1007/BF02947264.
Using the standard indirect immunofluorescent (IIF) technique two types of autoantibodies are detected in the sera of patients with vasculitic disorders. These are cytoplasmic or classical antineutrophil cytoplasmic antibody (cANCA) and perinuclear anti-neutrophil cytoplasmic antibody (pANCA). In order to resolve the problems associated with the detection of pANCA an immunocytochemical technique-alkaline phosphatase anti-alkaline phosphatase (APAAP) was developed and used to detect ANCA in various groups of patients. Comparison with the standard immunofluorescence method showed that the results correlated only when immunostaining patterns were of the cANCA type. Detection of pANCA by APAAP was more uncertain than by immunofluorescence because of the greater number of staining patterns seen. Interference from antinuclear antibodies (ANA) appeared to cause more problems with the APAAP technique and sera containing ANA were not distinguished from pANCA using either immunofluorescence or APAAP. In conclusion, it appears that the APPAP technique is not a reliable method of the screening of ANCA and that pANCA has several antigenic specificities.
运用标准间接免疫荧光（IIF）技术，在血管炎疾病患者的血清中检测到两种自身抗体。它们分别是胞质型或经典抗中性粒细胞胞质抗体（cANCA）和核周型抗中性粒细胞胞质抗体（pANCA）。为了解决与pANCA检测相关的问题，研发了一种免疫细胞化学技术——碱性磷酸酶抗碱性磷酸酶（APAAP），并用于检测各类患者群体中的ANCA。与标准免疫荧光法的比较表明，只有当免疫染色模式为cANCA型时，结果才具有相关性。由于观察到的染色模式数量更多，通过APAAP检测pANCA比通过免疫荧光检测更不确定。抗核抗体（ANA）的干扰似乎给APAAP技术带来了更多问题，使用免疫荧光或APAAP都无法区分含有ANA的血清和pANCA。总之，似乎APPAP技术不是筛查ANCA的可靠方法，并且pANCA具有多种抗原特异性。
