Fujita Y, Yamamuro T, Nakamura T, Kotani S, Ohtsuki C, Kokubo T
Department of Orthopaedic Surgery, Faculty of Medicine, Kyoto University, Japan.
J Biomed Mater Res. 1991 Aug;25(8):991-1003. doi: 10.1002/jbm.820250806.
Plates of calcite (CaCO3) were implanted in rabbit tibiae, and their biocompatibility and bonding ability to bone were studied. The plates were also implanted subfascially in rabbit muscle for 8 weeks, and changes on their surfaces in the body were examined. Contact microradiography and Giemsa surface stain demonstrated direct bonding between calcite and bone without interpositions. The average failure load of the interface between calcite and bone was 4.11 kg, indicating an adequate strength of bonding. However, a Ca-P-rich layer, which formed on the surfaces of other bioactive ceramics in vivo, was not detected by a scanning electron microscope-electron probe x-ray microanalyzer. Scanning electron micrographs of the surface of calcite implanted subfascially for 8 weeks showed marked degradation and a rough surface. However, the surface apatite layer was not detected by thin-film x-ray diffraction analysis and Fourier transform infrared reflection spectroscopy. Calcite is a biodegradable material that bonds to bone without a surface apatite layer. The mechanical bonding provided by the anchoring effect of the newly formed bone into the surface roughness of calcite is considered to be a major factor in calcite-bone bonding.
将方解石（CaCO₃）片植入兔胫骨中，研究其生物相容性及与骨的结合能力。这些片还被植入兔肌肉的筋膜下8周，检查其在体内表面的变化。接触微放射照相术和吉姆萨表面染色显示方解石与骨之间直接结合，无中间物。方解石与骨界面的平均破坏载荷为4.11千克，表明结合强度足够。然而，扫描电子显微镜 - 电子探针X射线微分析仪未检测到在体内其他生物活性陶瓷表面形成的富含钙磷的层。筋膜下植入8周的方解石表面的扫描电子显微镜照片显示出明显的降解和粗糙表面。然而，薄膜X射线衍射分析和傅里叶变换红外反射光谱未检测到表面磷灰石层。方解石是一种可生物降解的材料，在没有表面磷灰石层的情况下与骨结合。新形成的骨锚定到方解石表面粗糙度所提供的机械结合被认为是方解石与骨结合的主要因素。
