Wu Huan-Gan, Liu Hui-Rong, Tan Lin-Ying, Gong Ye-Jing, Shi Yin, Zhao Tian-Ping, Yi Yao, Yang Yun
Shanghai Research Institute of Acupuncture and Meridian, XuHui District, 200030, Shanghai, PR China.
Dig Dis Sci. 2007 Feb;52(2):379-84. doi: 10.1007/s10620-006-9561-y. Epub 2007 Jan 9.
The purpose of this study was to investigate the effect of electroacupuncture (EA) and moxibustion on promoting neutrophil apoptosis. A rat model of ulcerative colitis was established by immunological methods using human colonic mucosa as antigen. All rats were randomly assigned to the model control (MC) group, EA group, or herbs-partition moxibustion (HPM) group. Normal rats were used as the normal control (NC) group. Peripheral blood mononuclear cells (PBMCs) from all rats and circular neutrophils from NC rats were isolated and cultured. Circular neutrophils were incubated with cultured supernatants of PBMCs from the MC, NC, EA, and HPM groups, respectively. Neutrophil apoptosis and concentration of IL-1beta, IL-6, and TNF-alpha from induced cultured supernatants were detected by cell cytometry and ELISA, respectively. Compared with MC, HPM, and EA rats, mucosal inflammatory lesions abated remarkably. No hyperemia or edema was seen in the lamina propria, inflammatory cell infiltration decreased, neutrophil infiltration disappeared, and epithelial and crypt cells proliferated and repaired the ulceration of the mucosa. Neutrophil apoptosis was promoted. Concentrations of IL-1beta, IL-6, and TNF-alpha were decreased, respectively. We conclude that EA and HPM therapy can improve ulcerative colitis rats histologically, which may be due to promoting neutrophil apoptosis and down-regulating monocyte cytokines. EA and moxibustion are effective for treating ulcerative colitis.
本研究旨在探讨电针（EA）和艾灸对促进中性粒细胞凋亡的作用。以人结肠黏膜为抗原，采用免疫方法建立溃疡性结肠炎大鼠模型。将所有大鼠随机分为模型对照组（MC）、电针组或隔药灸（HPM）组。以正常大鼠作为正常对照组（NC）。分离并培养所有大鼠的外周血单个核细胞（PBMC）和NC大鼠的循环中性粒细胞。将循环中性粒细胞分别与MC、NC、EA和HPM组PBMC的培养上清液共同孵育。分别采用细胞流式术和ELISA检测诱导培养上清液中中性粒细胞凋亡情况以及IL-1β、IL-6和TNF-α的浓度。与MC、HPM和EA组大鼠相比，黏膜炎症损伤明显减轻。固有层未见充血或水肿，炎症细胞浸润减少，中性粒细胞浸润消失，上皮细胞和隐窝细胞增殖并修复黏膜溃疡。中性粒细胞凋亡得到促进。IL-1β、IL-6和TNF-α的浓度分别降低。我们得出结论，电针和隔药灸疗法可在组织学上改善溃疡性结肠炎大鼠，这可能是由于促进了中性粒细胞凋亡并下调了单核细胞细胞因子。电针和艾灸对治疗溃疡性结肠炎有效。
