Kosaka Tomoyuki, Nakayama Shunichi, Nakaya Keizo, Yoshino Sadazo, Furukawa Kensuke
Laboratory of Applied Microbiology, Faculty of Agriculture, Kyushu University, Japan.
Biosci Biotechnol Biochem. 2007 Jan;71(1):58-68. doi: 10.1271/bbb.60370. Epub 2007 Jan 7.
The solventogenic sol operon consisting of bld, ctfA, ctfB, and adc was cloned from Clostridium saccharoperbutylacetonicum strain N1-4. These genes share as high as 95-98% similarity with the corresponding sol genes of Clostridium beijerinckii NCIMB 8052. The N1-4 sol gene cluster was transcribed in a polycistronic manner under the control of two promoters, and its transcription was highly induced during solventogenesis. Strain DGN3-4, the degenerated strain derived from N1-4, maintained the sol genes, but transcription of the DGN3-4 sol operon was hardly induced during solventogenesis. A substance extracted from the culture supernatants of wild-type N1-4 allowed us to induce transcription of the sol operon in DGN3-4. These results suggest that the degeneration is caused by the incompetence of the induction mechanism of the sol operon, and that transcription might be under the control of a quorum-sensing mechanism.
从产糖丁酸梭菌N1-4菌株中克隆出由bld、ctfA、ctfB和adc组成的产溶剂梭操纵子。这些基因与拜氏梭菌NCIMB 8052的相应梭基因具有高达95%-98%的相似性。N1-4梭基因簇在两个启动子的控制下以多顺反子方式转录，并且其转录在溶剂生成过程中被高度诱导。源自N1-4的退化菌株DGN3-4保留了梭基因，但DGN3-4梭操纵子的转录在溶剂生成过程中几乎不被诱导。从野生型N1-4的培养上清液中提取的一种物质使我们能够诱导DGN3-4中梭操纵子的转录。这些结果表明，退化是由梭操纵子诱导机制的无能引起的，并且转录可能受群体感应机制的控制。
